Effects of Bucillamine on the Function of Human B Cell and T Cell.
- Author:
Sung Hwan PARK
1
;
Soon Ja LIM
;
Jun Ki MIN
;
Jae Ho PARK
;
Yeon Sik HONG
;
Sang Heon LEE
;
Chul Soo CHO
;
Ho Youn KIM
Author Information
1. Department of Internal Medicine, Catholic University Medical College, Kangnam St. Mary's Hoapital, Seoul, Korea.
- Publication Type:In Vitro ; Original Article ; Clinical Trial
- Keywords:
Bucillamine;
D-penicillamine;
Rheumatoid arthritis
- MeSH:
Arthritis, Rheumatoid;
B-Lymphocytes;
Cell Proliferation;
DNA;
Humans*;
Immunoglobulin M;
Interleukin-2;
Penicillamine;
Rheumatoid Factor;
Staphylococcus aureus;
T-Lymphocytes;
Tissue Donors
- From:The Journal of the Korean Rheumatism Association
1996;3(2):172-178
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: Bucillamine[N- (2-mercapto-2-methylpropionyl)-L-cysteine] (BUC) is a thiol compound that differs from D-pencillamine(DPC) in that it contains two free sulfhydryl groups. Clinical trials have suggested that the efficacy of BUC in rheumatoid arthritis(RA) is as effective as DPC, but the mechanism of action remains unclear. We therefore examined the effects of BUC on the in vitro function of human B cell and T cell in comparision to those of DPC. METHODS: The effect of BUC and DPC on Staphylococcus aureus Cowan 1 (SAC) induced IgM production by B cells from 11 healthy donors was examined. Phytohemagglutinin (PHA) induced proliferation and Interferon-r(IFN-r) and Interleukin-2 (IL-2) production by T cells was also examined. RESULTS: BUC and BUC-ID(SA981) suppressed the production of IgM at concentration of 0. 1-100 ug/ml, whereas DPC suppressed at concentration of 100 ug/ml. BUC and DPC inhibited PHA induced DNA synthesis of peripheral blood mononuclear cells(PBMCs) and T cell in a dose-dependent manner. DPC (10 ug/ml) significantly suppresed IFN-r production by PHA-stimulated T cells, but not suppressed IL-2 production, whereas BUC(10 ug/ml) not significantly suppressed IFN-r and IL-2 production. CONCLUSION: BUC has immunosuppressive effects inhibiting the function of B cells and T cell proliferation, whereas the action of DPC is targeted at T lymphocytes. BUC may be effective in rheumatoid factor positive RA patients who have not responded to treatment with DPC.
