CD20dim T Cell Expression in Rheumatoid Arthritis.
- Author:
Jong Wook LEE
1
;
Jung Woon LEE
;
Hyon Suk KIM
;
Oh Hun KWON
;
Soo Kon LEE
Author Information
1. Department of Clinical Pathology Yonsei University College of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
CD20dim T cell;
Rheumatoid arthritis
- MeSH:
Arthritis, Rheumatoid*;
B-Lymphocytes;
Humans;
Immunoglobulin G;
Immunophenotyping;
Reference Values;
T-Lymphocytes;
Tissue Donors
- From:The Journal of the Korean Rheumatism Association
1996;3(2):179-186
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: This study was designed to investigate CD20dim expression on T cells between healthy control subjects and rheumatoid arthritis patients and to investigate the association between CD20dim T cells and clinical manifestations in rheumatoid arthritis. METHODS: Peripheral bloods from twenty seven healthy controls and twenty six rheumatoid patients were evaluated by dual color immunophenotyping. We used IgGla and IgG2a for negative control, then, divided T cells and B cells on CD3/CD19 combination. And we evaluated two subpopulation CD5+CD20dim T cell (CD20dim T cell) and CD5+CD20bright cell(CD5 B cell) on CD5/20 combination for comparison. In order to correlate the CD20dim T cell datas with patient's clinical status, patients charateristcs such as age, disease duration, morning stiffness, rheumatoid factor(RF), and antinuclear antibody(ANA) were reviewed. RESULTS: The mean percent positivity of T cells(CD3) and B cells(CD19) in 27 healthy control donors was 63.2+/-7.7%, and 11.4+/-2.6 %. The mean percentages of CD20dim T cell and CD5 B cell(Leul) were 2.3+/-1.0% and 1.4+/-0.9% and upper limit of reference values of CD20dim T cells and CD5 B cells(Leul) that calculated from normal healthy controls were 4.3%0 and 3. 2 %. Compared with healthy controls, CD20dim T cells were significantly elevated in total patient groups(4.4+/-2.7%, p(0.01). and significantly increased more than upper limit of reference values in eleven of 26 patients. Compared with CD20dim T cells according to disease duration, disease duration of most patients(10/11) with elevated CD20dim T cell were less than 2 years. CONCLUSION: The results indicated that CD20dim T cells were expressed at low levels in healthy controls but elevated levels of CD20dim T cells in rheumatoid arthritis patients were observed and close relationship to CD20dim T cells expression and disease duration was noted.
