Real-Time PCR analysis of af4 and dek genes expression in acute promyelocytic leukemiat (15;17)patients.
- Author:
Hakan SAVLI
1
;
Sema SIRMA
;
Balint NAGY
;
Melih AKTAN
;
Guncag DINCOL
;
Zafer SALCIOGLU
;
Nazan SARPER
;
Ugur OZBEK
Author Information
1. Department of Medical Biology, Medical Faculty, University of Kocaeli, Kocaeli, Turkey. uozbek@istanbul.edu.tr
- Publication Type:Case Report
- Keywords:
af4;
APL;
dek;
Gene expression;
Real Time PCR
- MeSH:
Adolescent;
Adult;
Child;
Child, Preschool;
Chromosomal Proteins, Non-Histone/*genetics/metabolism;
Chromosomes, Human, Pair 15;
Chromosomes, Human, Pair 17;
DNA-Binding Proteins/*genetics/metabolism;
Down-Regulation;
Female;
Gene Expression;
Humans;
Leukemia, Promyelocytic, Acute/*genetics/metabolism;
Male;
Middle Aged;
Nuclear Proteins/*genetics/metabolism;
Oncogene Proteins/*genetics/metabolism;
Polymerase Chain Reaction;
RNA, Messenger/analysis/metabolism;
Translocation, Genetic;
Up-Regulation
- From:Experimental & Molecular Medicine
2004;36(3):279-282
- CountryRepublic of Korea
- Language:English
-
Abstract:
Among several newly identified oncogenes, dek and af4 are attractive targets for researchers interested with leukemia. In this study quantitative Real-Time RT-PCR technique was used to define alterations in expression of dek and af4 genes associated with acute promyelocytic leukaemia (APL) t (15; 17). RNA samples obtained from bone marrow aspirates of fourteen APL patients, cDNA portions were labelled with Syber Green 1 dye and LightCycler analysis have been performed. Expression changes in patients were found not significant in comparison to healthy donors for af4 (P=0.192) and dek (P= 0.0895). We suggest that af4 gene may have a role in leukomogenesis restricted to lymphoblastic lineage; also further studies must carry on with a larger series of patients in order to understand the relationship between the dek gene and APL. Our study was the first attempt for analysing dek and af4 genes in APL t (15; 17) patients by quantitative Real-Time RT-PCR. This rapid and sensitive method could be used to screen these genes in different types of leukaemia.
