Time Course Study of Cytokine mRNA Expression in LPS-Stimulated Porcine Alveolar Macrophages.
- Author:
In Soo CHOI
1
;
Na Ri SHIN
;
Sung Jae SHIN
;
Deog Yong LEE
;
Young Wook CHO
;
Han Sang YOO
Author Information
1. Department of Infectious Diseases, College of Veterinary Medicine and School of Agricultural Biotechnology,Seoul National University, Suwon 441-744, Korea. yoohs@plaza.snu.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Kinetics;
porcine;
cytokine expression;
alveolar macrophages;
LPS-stimulation
- MeSH:
Animals;
Cells, Cultured;
Cytokines/*genetics;
Female;
Gene Expression Profiling;
Gene Expression Regulation/*drug effects;
Granulocyte-Macrophage Colony-Stimulating Factor/genetics;
Interferon Type II/genetics;
Interleukins/genetics;
Kinetics;
Lipopolysaccharides/*pharmacology;
Macrophages, Alveolar/*drug effects/*metabolism;
RNA, Messenger/genetics/metabolism;
Reverse Transcriptase Polymerase Chain Reaction;
Sensitivity and Specificity;
Swine/*genetics
- From:Journal of Veterinary Science
2002;3(2):97-101
- CountryRepublic of Korea
- Language:English
-
Abstract:
The kinetics of cytokine mRNA expression was studied in porcine alveolar macrophages using an RT-PCR assay. The expression levels of IFN- gamma, IL-2, IL-4, IL-6, GM-CSF, IL-12 p35, and IL-12 p40 were examined after 2, 4, 14, 24, 48, and 72 h of incubation in unstimulated control and LPS-stimulated cells. The expression contents of IFN-gamma, IL-2, and IL-4 were not detected in both unstimulated and LPS-stimulated cells. On the other hand, the expression levels of IL-6, GM-CSF, and IL-12 in LPS-stimulated cells were almost always higher than those in control cells. Among those cytokines, IL-6 exhibited the predominant expression, and GM-CSF, IL-12 p40, and IL-12 p35 followed in the descending order. The times to reach the peak expression levels for IL-6, and GM-CSF, IL-12 p35, and IL-12 p40 were 14, and 24 h, respectively. After reaching the peak expression point, the expression levels of IL-6, GM-CSF, and IL-12 p40 reduced to the baseline by 72 h after stimulation, however, IL-12 p35 still kept a substantial expression by the same time. This study demonstrates that porcine alveolar macrophages primarily respond to express IL-6, GM-CSF, and IL-12 by LPS-stimulation and have a cytokine-specific expression profile during the stimulation time.
