Simultaneous Determination of Various Macrolides by Liquid Chromatography/Mass Spectrometry.
- Author:
Youn Hwan HWANG
1
;
Jong Hwan LIM
;
Byung Kwon PARK
;
Hyo In YUN
Author Information
1. Division of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Chungnam National University, Korea. hiyun@hanbat.chungnam.ac.kr
- Publication Type:Original Article
- Keywords:
simultaneous determination;
liquid chromat ography/mass spectrometry;
macrolides antibiotics
- MeSH:
Anti-Bacterial Agents/*analysis;
Chromatography, Liquid/*methods;
Diterpenes/*analysis;
Erythromycin/*analysis;
Mass Spectrometry/*methods;
Molecular Structure;
Roxithromycin/*analysis;
Sensitivity and Specificity;
Tylosin/*analysis;
Veterinary Medicine
- From:Journal of Veterinary Science
2002;3(2):103-108
- CountryRepublic of Korea
- Language:English
-
Abstract:
Macrolides are frequently used in veterinary medicine as therapeutic and preventive agents for various diseases. It is difficult to determine macrolides simultaneously with conventional methods due to their similar structures. A simultaneous analysis for erythromycin, roxithromycin, tiamulin and tylosin with LC/MS has been developed. Separation was performed on C18 reversed phase column. Mobile phase was gradiently flowed with 10 mM ammonium acetate and methanol. The mass spectrometer was run in the positive mode and selective ion monitoring mode. The molecular ions were [M+H]+ form at m/z 837.5 for erythromycin, at m/z 859.5 for roxithromycin, at m/z 494.2 for tiamulin and at m/z 916.7 for tylosin. Limits of detection were in the range from 0.001 to 0.01 microgram/g lower than their MRLs.
