The Pattern of Expression of Phospholipase C-gamma in Benign and Malignant Tumors of the Breast.
- Author:
Hee Joung KIM
1
;
Ki Wook CHUNG
;
Sung Won KIM
;
Byung In MOON
;
Soo Jung AHN
;
Dong Young NOH
;
Keun Young YOO
;
Kuk Jin CHOE
Author Information
1. Department of Surgery, Seoul National University College of Medicine, Korea. dynoh@plaza.snu.ac.kr
- Publication Type:Original Article
- Keywords:
PLC-gamma;
Fibroadenoma;
Breast cancer
- MeSH:
Breast Neoplasms;
Breast*;
Densitometry;
Fibroadenoma;
Phospholipase C gamma;
Phospholipases*;
Protein-Tyrosine Kinases;
Signal Transduction;
Type C Phospholipases
- From:Journal of the Korean Surgical Society
2002;62(6):463-467
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The activation of phospholipase C (PLC) is one of the early events in various growth processes, including malignant transformation. Among PLC-isozymes, PLC-gamma is activated through direct interaction with growth factor receptor tyrosine kinase. In this study, we evaluated the patterns of PLC-gamma expression in benign and malignant tumors of the breast and compared the patterns with their normal counterpart. METHODS: Using immunoblot assay, we evaluated the patterns of expression in PLC-gamma in 20 breast cancer tissues, 13 fibroadenoma tissues, and normal tissues. The level of expression was analyzed by densitometry. RESULTS: All of 20 breast cancer tissues and 13 fibroadenoma tissues showed overexpression of PLC-gamma when compared with their normal counterparts. The level of the PLC-gamma expression was 3.9-fold and 17.3-fold higher in fibroadenomas and breastcancers, respectively, than in normal tissues. CONCLUSION: The result suggested that the level of PLC-gamma expression increases as the normal tissue undergoes progression to fibroadenoma, and finally to carcinoma. The pattern of expression of PLC-gamma in breast tissue implies that the PLC-gamma-mediated signal transduction may play a significant role in the progression of breast cancer from normal tissue.
