Development of Tetracycline-regulated Adenovirus Expression Vector System.
- Author:
Kyung Hwa SON
1
;
Seung Hoon LEE
;
Jong Sik KIM
;
Jung Joo CHOI
;
Je Ho LEE
Author Information
1. Center for Clinical Research, Samsung Biomedical Research Institute, Samsung Medical Center, 50 Ilwon-dong, Kangnam-Ku, Seoul, Korea. jeholee@samsung.co.kr
- Publication Type:Original Article
- Keywords:
Tetracycline-regulated adenovirus;
Expression vector
- MeSH:
Adenoviridae*;
Cell Physiological Phenomena;
Gene Expression;
Genetic Therapy;
Tetracycline
- From:Journal of Genetic Medicine
1999;3(1):33-36
- CountryRepublic of Korea
- Language:English
-
Abstract:
Recombinant adenovirus vector systems with strong promoters have been used to achieve high level production of recombinant protein. However, this overexpression system cause some problems such as disturbance of cell physiology and increment of cellular toxicity. Here, we showed a tetracycline-regulated adenovirus expression vector system. Our results showed that the expression level of transgene(p-53) was high and easily regulated by tetracycline. In addition, the maximal gene expresion level of the tetracycline-controlled gene expression system was higher than that of the wild type CMV promoter system. Therefore, tetracycline-regulated adenoviral vector system could be applicable for regulatory high-level expression of toxic gene. Also, this system will be useful for functional studies and gene therapy.
