Nitric Oxide(NO) in Inflammatory Arthritis.
- Author:
Sang Cheol BAE
1
;
Dong Yook KIM
;
Tae Hwan KIM
;
Jae Bum JUN
;
Sung Soo JUNG
;
In Hong LEE
;
Dae Hyun YOO
;
Seong Yoon KIM
;
Eun Young LEE
;
Sung Yeoul CHANG
Author Information
1. Department of Internal Medicine, Rheumatism Center, College of Medicine, Hanyang University, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Chondrocyte;
Inflammatory Arthritis;
Nitric Oxide;
Nitrite;
Rheumatoid Arthritis;
Synoviocyte
- MeSH:
Antirheumatic Agents;
Arginine;
Arthritis*;
Arthritis, Rheumatoid;
Autoimmunity;
Blood Cells;
Chondrocytes;
Cycloheximide;
Cytokines;
Dexamethasone;
Gold Sodium Thiomalate;
Humans;
Indomethacin;
Inflammation;
Insulin-Like Growth Factor I;
Metabolism;
Methotrexate;
Neurotransmitter Agents;
Nitric Oxide;
omega-N-Methylarginine;
Synovial Fluid;
Transforming Growth Factor beta
- From:Korean Journal of Medicine
1997;52(1):32-41
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVES:Nitric Oxide(NO) is a toxic, inorganic, gaseous free radical produced during the metabolism of L-Arginine by NO synthase(NOS). It has been implicated in a rapidly growing number of physiological and pathophysiological processes such as cytotoxic effects against microbes and tumor cells, blood vessel dilation and neurotransmitter. Recently there is growing evidence implicating NO in immune regulation, inflammation, autoimmunity, and arthritis. We performed this study to determine a role for nitric oxide in inflammatory arthritis especially rheumatoid arthritis(RA). METHODS: We measured (1) the concentrations of nitrite, a breakdown product of nitric oxide, in serum and synovial fluid from patients with RA and osteoarthritis(OA) and in the serum of controls (2) the concentrations of nitrite in the supernatant of cultured synovial tissue with RA and OA and (3) determined whether human chondrocytes and synoviocytes can synthesize nitric oxide and if so, how production is regulated by cytokines and antirheumatic drugs. RESULTS: 1) Serum nitrite concentrations in patients with RA and OA were higher than in controls. In both disease groups synovial fluid nitrite was higher than serum nitrite. Serum and synovial fluid nitrite concenrations in RA were higher than those in OA. However, those findings are not statistically significant. 2) Although these findings are not statistically significant, the concentration of nitrite in the supernatant of cultured synavial tissue with RA was higher than that in OA. 3) IL-1beta and TNF-alpah induced the biosynthesis of NO by chondrocytes and synoviocytes. IGF-1 and TGF-beta failed to provoke the production of NO. The biosynthesis of NO required an induction period of approximately 6 hours and was inhibited by L-NMMA and cycloheximide. Dexamethasone, indomethacin, gold sodium thiomalate and methotrexate had no effect on the induction of NO biosynthesis. CONCLUSION: These results suggest a role for nitric oxide as an inflommatory mediator in inflammatory arthritis.
