The Association between Pneumoconiosis and Genetic Polymorphism of GSTM1, GSTT1, GSTP1, NAT2, CYP2E1 and CYP1A1.
- Author:
Cha Jae OH
1
;
Moon Young HWANG
;
Kang Jin LEE
;
Hoo Rak LEE
;
Byung Mann CHO
Author Information
1. Department of Preventive and Occupational Medicine, College of Medicine, Pusan National University, Occupational Health Center, Hyundai Motor Company, Korea. bmcho@hanmail.net
- Publication Type:Original Article
- Keywords:
Pneumoconiosis;
Genetic polymorphism;
GSTM1;
GSTT1;
GSTP1;
NAT2;
CYP2E1;
CYP1A1
- MeSH:
Age of Onset;
Cytochrome P-450 CYP1A1*;
Cytochrome P-450 CYP2E1*;
Cytochrome P-450 Enzyme System;
DNA;
Genotype;
Glutathione Transferase;
Humans;
Logistic Models;
Multiplex Polymerase Chain Reaction;
Odds Ratio;
Pneumoconiosis*;
Polymorphism, Genetic*;
Questionnaires;
Risk Factors;
Smoke;
Smoking
- From:Korean Journal of Occupational and Environmental Medicine
2005;17(2):85-94
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVES: To investigate effects of genetic polymorphism of glutathione S-transferase M1 (GSTM1), glutathione S-transferase T1 (GSTT1), glutathione S-transferase P1 (GSTP1), N-acetyltransferase (NAT2), cytochrome P450 2E1 (CYP2E1) and cytochrome P450 1A1 (CYP1A1) on pneumoconiosis. METHODS: Eighty-five pneumoconiosis patients and 122 age and sex matched healthy controls were enrolled. Direct interview and standard questionnaire were conducted and the genotypes of GSTM1, GSTT1, GSTP1, NAT2, CYP2E1 and CYP1A1 were investigated using multiplex PCR or PCR-RFLP methods with DNA extracted from venous blood. The relationship was investigated between the severity of pneumoconiosis and the polymorphism of GSTM1, GSTT1, GSTP1, NAT2, CYP2E1 and CYP1A1, and also with various environmental factors including smoking. RESULTS: We observed a significantly higher rate of genetic polymorphism in pneumoconiosis patients than in normal subjects. The odds ratio (95% CI) of NAT2 was 2.09 (1.19-3.68). In addition, smoking was related significantly with pneumoconiosis (OR 2.89, 95% CI 1.40-5.95). In multiple logistic regression analyses, NAT2 and smoking were significant risk factors for the development of pneumoconiosis (OR 1.84, 95% CI 1.00-3.37; OR 2.98, 95% CI 1.40-6.35, respectively). The age of onset of the disease and smoking were significantly related with moderate or severe pneumoconiosis (OR 0.91, 95% CI 0.83-0.99; OR 6.94, 95% CI 1.54-31.30, respectively). However there was no significant difference between the rate of genetic polymorphism of GSTM1, GSTT1, GSTP1, CYP2E1 and CYP1A1 in the two groups. CONCLUSION: NAT2 genetic polymorphism was higher in pneumoconiosis patients than in normal subjects. The age of onset of the disease and smoking were significantly related with pneumoconiosis. However, the genetic polymorphism of GSTM1, GSTT1, GSTP1, CYP2E1 and CYP1A1 was not related with development or severity of pneumoconiosis.
