Effects of the immobilization of heparin and rhPDGF-BB to titanium surfaces for the enhancement of osteoblastic functions and anti-inflammation.
- Author:
Jung Bo HUH
1
;
Jeong Yol LEE
;
Kyung Lae LEE
;
Sung Eun KIM
;
Mi Jung YUN
;
Ji Suk SHIM
;
June Sung SHIM
;
Sang Wan SHIN
Author Information
- Publication Type:Original Article
- Keywords: Titanium; Heparin; rhPDGF-BB; Anti-inflammation; Osteoblastic function
- MeSH: Alkaline Phosphatase; Calcium; Cell Proliferation; Dopamine; Electrons; Heparin; Humans; Immobilization; Inflammation; Interleukin-6; Kinetics; Osteoblasts; Platelet-Derived Growth Factor; Proto-Oncogene Proteins c-sis; RNA, Messenger; Titanium; Tumor Necrosis Factor-alpha
- From:The Journal of Advanced Prosthodontics 2011;3(3):152-160
- CountryRepublic of Korea
- Language:English
- Abstract: PURPOSE: This study was to investigate the effects of recombinant human platelet-derived growth factor (rhPDGF-BB) and heparin to titanium surfaces for enhancement of osteoblastic functions and inhibition of inflammation activity. MATERIALS AND METHODS: The anodized titanium discs, not coated with any material, were used as a control group. In heparinized- Ti group, dopamine was anchored to the surface of Ti substrates, and coated with heparin. In PDGF-Ti group, rhPDGF-BB was immobilized onto heparinized Ti surface. The surface morphologies were investigated by the scanning electron microscope in each group. The release kinetics of rhPDGF-BB were analyzed, and cytotoxicity tests for each group were conducted. The biocompatibilities were characterized by measuring cell proliferation, alkaline phosphatase activity, and calcium deposition using MG-63 cells. Statistical comparisons were carried out by one-way ANOVA tests. Differences were considered statistically significant at *P<.05 and **P<.001. RESULTS: The combination of rhPDGF-BB and heparin stimulated alkaline phosphatase activity and OCN mRNA expression in osteoblastic cells (*P<.05 and **P<.001). MG-63 cells grown on PDGF-Ti had significantly higher amounts of calcium deposition than those grown on anodized Ti (**P<.001). Heparinized Ti was more anti-inflammatory compared to anodized Ti, when exposed to lipopolysaccharide using the transcript levels of TNF-alpha and IL-6 of proinflammatory cytokine (*P<.05 and **P<.001). CONCLUSION: The result of this study demonstrated that the incorporation of rhPDGF-BB and heparin onto Ti surface enhanced osteoblastic functions and inhibited inflammation.
