Mutation and overexpression of p53 as a prognostic factor in canine mammary tumors.
- Author:
Chung Ho LEE
1
;
Wan Hee KIM
;
Ji Hey LIM
;
Min Soo KANG
;
Dae Yong KIM
;
Oh Kyeong KWEON
Author Information
1. Department of Veterinary Surgery, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea. Koreaohkweon@snu.ac.kr
- Publication Type:Original Article
- Keywords:
canine;
mutation;
overexpression;
p53;
prognosis
- MeSH:
Animals;
Antibodies, Antinuclear/metabolism;
Antibodies, Monoclonal/metabolism;
Blotting, Western/veterinary;
Dog Diseases/genetics/*metabolism;
Dogs;
Female;
Genes, p53/*genetics;
Immunohistochemistry/veterinary;
Ki-67 Antigen/metabolism;
Mammary Neoplasms, Animal/genetics/*metabolism;
*Mutation;
Predictive Value of Tests;
Proportional Hazards Models;
Tumor Suppressor Protein p53/*biosynthesis/genetics
- From:Journal of Veterinary Science
2004;5(1):63-69
- CountryRepublic of Korea
- Language:English
-
Abstract:
We concentrated ourselves to evaluate the prognostic significance of the p53 gene mutations, its protein expression and MIB-1 index as a proliferative marker in canine mammary tumors. In the present study, a total of 20 cases were examined, among which there were 5 malignant mixed tumors, 4 mammary gland adenocarcinomas, 1 papillary adenocarcinoma, 8 benign mixed tumors and 2 mammary gland adenomas. Positive immunostaining for p53 with PAb240 antibody was found in 2 benign (20%) and 3 malignant (30%) tumors. However, PAb421 antibody did not give positive result at all. In Western blot analysis, the p53 expression in benign and malignant tumors was detected in 4 and 3 cases, respectively. p53 mutations were found in 6 cases out of the cases with detected p53 protein expression. The MIB-1 index in benign and malignant tumors were 17.6+/-20.8% and 29.0+/-27.2%, respectively and there was no significant difference between tumor types. There was a significant correlation between p53 mutations and p53 overexpression (correlation coefficient = 0.5, p < 0.05). In Kaplan-Meier survival analysis, the p53 index was associated with significantly shortened survival time (p < 0.01). In multivariate analysis, p53 overexpression was only an independent factor for indicator of worse prognosis in canine mammary tumors (p = 0.01). These results demonstrated that p53 gene mutations and protein overexpression using the PAb240 anti-p53 antibody were useful predictors of increased malignant potential and poor prognosis in canine mammary tumors.
