Blood Brain Barrier and Brain Tissue Injury by Gd-DTPA in Uremia-induced Rabbits.
10.3348/jkrs.1996.35.6.837
- Author:
Sun Seob CHOI
1
;
Yong Chul LEE
;
Kun Sang KIM
;
Choong Gi EUN
;
Yeong Il YANG
;
Ki Yeong HUH
;
Jin Yeong HAN
Author Information
1. Department of Diagnostic Radiology, Dong-A University College of Medicine, Korea.
- Publication Type:Original Article
- Keywords:
Animals;
Contrast media, toxioity;
Brain injuries;
Gadotinium
- MeSH:
Animals;
Basement Membrane;
Blood-Brain Barrier*;
Brain Injuries;
Brain*;
Demyelinating Diseases;
Edema;
Endothelial Cells;
Erythrocytes;
Gadolinium DTPA*;
Glial Fibrillary Acidic Protein;
Ligation;
Magnetic Resonance Imaging;
Myelin Sheath;
Neurons;
Rabbits*;
Renal Artery;
Tight Junctions;
Veins
- From:Journal of the Korean Radiological Society
1996;35(6):837-846
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: An experimental study was carried out to evaluate the morphological changes in the blood brain barrier and neighbouring brain tissue caused by Gd-DTPA in uremia-induced rabbits. MATERIALS AND METHODS: Bilateral renal arteries and veins of ten rabbits were ligated. Gd-DTPA(0.2 mmol/kg) was intravenously injectedinto seven rabbits immediately after ligation. After MRI, they were sacrificed 2 or 3 days after ligation in orderto observe light and electron microscopic changes in the blood brain barrier and brain tissue. RESULTS: MRIfindings were normal, except for enhancement of the superior and inferior sagittal sinuses on T1 weighted imagesin uremia-induced rabbits injected with Gd-DTPA. On light microscopic examination, these rabbits showed perivascular edema and glial fibrillary acidic protein expression ; electron microscopic examination showed separation of tight junctions of endothelial cells, duplication/rarefaction of basal lamina, increased lysosomes of neurons with neuronal death, demyelination of myelin, and extravasation of red blood cells. Uremia-induced rabbits injected with Gd-DTPA showed more severe changes than those without Gd-DTPA injection. CONCLUSION: Injuries to the blood brain barrier and neighbouring brain tissue were aggravated by Gd-DTPA administration inuremia-induced rabbits. These findings appear to be associated with the neurotoxicity of Gd-DTPA.
