Anti-Allergic Effects of Aloe Glycoprotein (NY945) on Ovalbumin-Induced Airway Allergy in the Guinea Pig.
- Author:
Ja Bock YUN
1
;
Weon Jin SEONG
;
Myung Hee CHUNG
;
Yang Gi MIN
Author Information
1. Department of Otolaryngology, Sanggye Paik Hospital, Inje University College of Medicine, Seoul, Korea. yun9669@dreamwiz.com
- Publication Type:Original Article
- Keywords:
Aloe vera;
Anti-allergic effect;
Nasal capillary permeability;
Total a irway resistance;
Ovalbumin
- MeSH:
Airway Resistance;
Aloe*;
Animals;
Eosinophils;
Female;
Glycoproteins*;
Guinea Pigs*;
Guinea*;
Histamine;
Histamine Release;
Humans;
Hypersensitivity*;
Immunoglobulin E;
Inflammation;
Inhalation;
Injections, Intraperitoneal;
Mast Cells;
Nasal Lavage Fluid;
Nasal Mucosa;
Nebulizers and Vaporizers;
Ovalbumin;
Ovum;
Permeability;
Ultrasonics;
Wound Healing
- From:Journal of Rhinology
2002;9(1, 2):16-23
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND AND OBJECTIVES: Aloe is one of the well known substances to decrease inflammation and promote wound healing. Although little is known about its mechanism, recent studies suggest that aloe vera extracts may have the anti-allergic effect by inhibiting the mediator release evoked by mast cell activation. Therefore, we were to investigate the effect of NY945, a glycoprotein fraction of Aloe vera, on nasal mucosa of the ovalbumin (OVA) sensitized guinea pigs. Materials and Methods: Female Dunkin-Hartley guinea pigs were sensitized with intraperitoneal injection and subsequent inhalation of OVA. NY945 was injected intraperitonealy before intranasal challenge of OVA with an ultrasonic nebulizer. Airway resistance, mucosal permeability, histamine concentration in the nasal lavage fluid, the number of mast cells and eosinophils in nasal mucosa and serum level of OVA -specific IgE were evaluated. RESULTS: NY945 effectively inhibited respiratory airway resistance in the sensitized guinea pigs. The concentration of dye into nasal lavage fluid was rapidly increased after OVA instillation and NY945 inhibited the OVA -induced microvascular dye leakage. OVA inhalation to sensitized guinea pig resulted in prominent increase of histamine concentration in the nasal lavage fluid. This response was inhibited by intraperitoneal NY945 injection. It also inhibited accumulation of eosinophils in the nasal mucosa in sensitized guinea pigs. However, there was no change in the serum OVA -specific IgE level between the NY945 treated and control group. CONCLUSION: The data suggest that NY945 inhibits early allergic reaction via inhibition of histamine release and eosinophil infiltration.
