Influence of the vitrification solution on the angiogenic factors in vitrificated mouse ovarian tissue.
10.5468/ogs.2013.56.6.382
- Author:
Won Jun CHOI
1
;
Ji Hye LEE
;
Mi Hyun PARK
;
In Young CHOI
;
Ji Kwon PARK
;
Jeong Kyu SHIN
;
Soon Ae LEE
;
Won Young PAIK
;
Jong Hak LEE
Author Information
1. Department of Obstetrics and Gynecology, Gyeongsang National University School of Medicine, Jinju, Korea. wypaik@gnu.ac.kr
- Publication Type:Original Article
- Keywords:
Angiopoietin-2;
Ovary;
Vascular endothelial growth factor A;
Vitrification
- MeSH:
Angiopoietin-2;
Animals;
Blotting, Western;
Cryopreservation;
Dimethyl Sulfoxide;
Female;
Methods;
Mice*;
Mice, Inbred ICR;
Ovary;
Reverse Transcriptase Polymerase Chain Reaction;
RNA, Messenger;
Vascular Endothelial Growth Factor A;
Vitrification*
- From:Obstetrics & Gynecology Science
2013;56(6):382-388
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: To investigate the effect of the dimethyl sulfoxide (DMSO) and EFS-40 during vitrification on the expression of angiogenic factors in vitrified mouse ovarian tissue. METHODS: The ovarian tissues were obtained from 5 or 6 weeks aged ICR mouse. Ovarian tissues were divided into four groups: ovarian tissue without cryopreservation (control, group I), ovarian tissue vitrified with 15% DMSO (group II), ovarian tissue vitrified with EFS-40 (group III), and ovarian tissue slowly frozen with 10% DMSO (group IV). Thawing was carried out at room temperature. Levels of messenger RNA (mRNA) and protein for vascular endothelial growth factor-A (VEGF-A) and angiopoietin-2 (Angpt-2) were checked in ovarian tissues of four groups recovered on day 7 after cryopreservation. Reverse transcription-polymerase chain reaction and Western blot analysis were used to identify the levels of angiogenic factors in mouse ovarian tissues. RESULTS: Levels of mRNA and protein for VEGF-A and Angpt-2 were significantly decreased in cryopreserved group (group II, III and IV) than control group (group I) (P< 0.05). The significant differences of levels of mRNA and protein for VEGF-A and Angpt-2 between cryopreservation methods were observed (P< 0.05). Group III showed highest expression of mRNA and protein for VEFG-A and Angpt-2 than other cryopreservation groups (P< 0.05). CONCLUSION: These findings suggest that EFS-40 is more efficient vitrification solution for preservation of angiogenic factors than 15% DMSO during vitrification of mouse ovarian tissue. Future studies should investigate to improve the vitrification solution for ovarian tissue vitrification.