Evaluation of Serum CTX and Osteocalcin Using Elecsys 2010.
- Author:
Tong Kil JUNG
1
;
Han Gil KIM
;
Hyun Sik CHOI
;
Nan Young LEE
;
Sin Goo PARK
;
Kyung Eun SONG
Author Information
1. Department of Clinical Pathology, School of Medicine, Kyungpook National University, Daegu, Korea.
- Publication Type:Original Article
- Keywords:
Serum bone-derived degradation products of type I collagen C-telopeptide;
S-CTX;
Serum osteocalcin;
Urine deoxypyridinoline;
DPD
- MeSH:
Bone Resorption;
Collagen Type I;
Humans;
Osteocalcin*;
Osteogenesis
- From:Korean Journal of Clinical Pathology
2001;21(6):459-464
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: In contrast with bone formation markers, most of available indices of bone resorption are urine markers and show relatively high degree of variability. The serum resorption assay has therefore been developed. We evaluated serum bone-derived degradation products of type I collagen C-telopeptide (s-CTX) and serum osteocalcin by Elecsys 2010 (Hitachi Boehringer Mannheim, Tokyo, Japan). METHODS: For 18 healthy controls, 15 osteopenic and 7 osteoporotic patients samples, serum CTX and serum osteocalcin were measured by Elecsys 2010 using -CrossLaps/serum (Roche Diagnostic Corp., Indianapolis, USA) kit and N-MID Osteocalcin (Roche Diagnostic Corp. kit, respectively. DPD by Immulite (Diagnostic Products Corp., LA, USA) using Pyrilinks-D(TM) (Diagnostic Products Corp.) kit and serum osteocalcin for correlation by Gamma counter (Hewlett Packard, Meriden, USA) using ELSA-OSTEO (CIS, Cedex, France) kit were measured. RESULTS: The within-run and between-run coefficient of variation (CV) values of s-CTX were 6.41% and 6% in low concentrations and 3.84% and 7% in high concentrations, respectively. The within-run and between-run CV values of serum osteocalcin were 2.21% and 6% in low concentrations and 1.25% and 3% in high concentrations, respectively. The dilution recovery of s-CTX and serum osteocalcin was 100-169% (mean, 134%) and 80-138% (mean, 104%), respectively. S-CTX and DPD (R=0.369, P=0.019), and serum osteocalcin by Elecsys 2010 and RIA (R=0.889, P<0.001) showed positive correlations, respectively. CONCLUSTIONS: S-CTX and serum osteocalcin by Elecsys 2010 exhibits good analytical performance and correlate with DPD and serum osteocalcin by RIA, respectively. Therefore, these may replace DPD and serum osteocalcin by RIA and can be used for bone resorption and formation markers, respectively.
