Regulation of MAPK Activity by Seizure-induced MKP-1 in Rat Hippocampus.
- Author:
Bum Hee YU
1
;
Ung Gu KANG
;
Yong Min AHN
;
Sun Ju CHUNG
;
Song Hee JEON
;
Joo Bae PARK
;
Yong Sik KIM
Author Information
1. Department of Psychiatry Sung-Kyun-Kwan University College of Medicine, Suwon, Korea.
- Publication Type:Original Article
- Keywords:
MKP-1;
MAPK;
Electroconvulsive shock;
Kainic acid;
Hippocampus
- MeSH:
Animals;
Electroshock;
Hippocampus*;
Kainic Acid;
Phosphorylation;
Rats*;
Seizures
- From:Journal of Korean Neuropsychiatric Association
1999;38(4):873-880
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVES: Both electroconvulsive shock(ECS) and kainic acid-induced seizures activate mitogenactivated protein kinases(MAPKs)in rat hippocampus. They can also induce the expression of MAPK phosphatase-1(MKP-1)in rat hippocampus. MKP-1 is known as a specific MAPK deactivator. This study aimed to elucidate the role of MKP-1 in the deactivation of MAPKs in rat hippocampus. METHODS: In order to induce MKP-1 in the hippocampus, ECS was given to the rats. At the time points when MKP-1 was sufficiently induced, the second ECS was given to them and the subsequent phosphorylation or activation of MAPKs were measured in the hippocampus. A second group of rats were injected with kainic acid and the relationship between MKP-1 expression and MAPK phosphorylation was examined in their hippocampi. RESULTS: The expression of MKP-1 did not influence the phosphorylation or activation of MAPKs following ECS in rat hippocampus. Kainic acid-induced expression of MKP-1 did not significantly reduce the phosphorylation of MAPKs. CONCLUSION: MKP-1 did not play a significant role in the deactivation of MAPKs which were activated by ECS or kainic acid in rat hippocampus.
