Effect of glucose on the expression of c-myc gene in cultured RINm5F cell.
10.3346/jkms.1991.6.3.246
- Author:
Sung Woon KIM
1
;
In Myung YANG
;
Jin Woo KIM
;
Young Seol KIM
;
Kwang Won KIM
;
Young Kil CHOI
;
Kwang Sik SUH
Author Information
1. Department of Internal Medicine, Kyung Hee University School of Medicine, Seoul, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
c-myc;
RINm5F cell;
glucose effect
- MeSH:
Animals;
Cell Line;
Gene Expression Regulation, Neoplastic/drug effects;
Genes, myc/*drug effects;
Glucose/*pharmacology;
Insulinoma/*genetics;
Pancreatic Neoplasms/*genetics;
Rats;
Tumor Cells, Cultured
- From:Journal of Korean Medical Science
1991;6(3):246-250
- CountryRepublic of Korea
- Language:English
-
Abstract:
The study was designed to examine the effect of glucose on the expression of c-myc gene in cultured RINm5F cells. After monolayer culture was established in RPMI 1640 media supplemented with 10% fetal calf serum (FCS), the cells were cultured in various concentrations of glucose and 1 or 10% FCS for another 24 hours. A mRNA was extracted from the cultured cells by a single step method, and Northern analysis was done to detect RNA band. A 0.5 kilobase single band was detected as c-myc mRNA. The expression of c-myc gene mRNA was reduced with increased concentration of glucose with 1% FCS. However, supplementation of 10% FCS abolished the effect of glucose on expression of c-myc gene. These findings suggested that glucose in conjunction with other growth promoting factors played an important role in expression of oncogene and cell growth in RINm5F cells.
