Protective Effect of Catechin on Apoptosis of the Lens Epithelium in Rats with N-methyl-N-nitrosourea-induced Cataracts.
10.3341/kjo.2010.24.2.101
- Author:
Sung Min LEE
1
;
Il Gyu KO
;
Sung Eun KIM
;
Dong Hee KIM
;
Byung Nam KANG
Author Information
1. Department of Ophthalmology, Myongji Hospital, Kwandong University College of Medicine, Goyang, Korea. bnkang@kd.ac.kr
- Publication Type:Original Article
- Keywords:
Apoptosis;
Cataract;
Catechin;
Crystalline lens;
Epithelial cells;
Rats
- MeSH:
Analysis of Variance;
Animals;
Animals, Newborn;
Apoptosis/*drug effects;
Blotting, Western;
Caspase 3/metabolism;
Cataract/chemically induced/*drug therapy;
Catechin/*pharmacology;
Immunoenzyme Techniques;
In Situ Nick-End Labeling;
Lens, Crystalline/*drug effects;
Random Allocation;
Rats;
Rats, Sprague-Dawley
- From:Korean Journal of Ophthalmology
2010;24(2):101-107
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: To investigate the effect of catechin on apoptotic cell death in the lens epithelium of rats with cataract. METHODS: Cataract was induced by intraperitoneal injection of 100 mg/kg N-methyl-N-nitrosourea (MNU) to ten day-old Sprague-Dawley rats. The neonatal rats were randomly divided into five groups (n=15 in each group): a control group, and four cataract-induction groups, treated with either 0, 50, 100, 200 mg/kg catechin. We performed slit-lamp biomicroscopic analysis, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay, Western-blot for Bcl-2 and Bax, and immunohistochemistry for caspase-3. RESULTS: Apoptotic cell death in lens epithelial cells that increased following cataract formation in rats was suppressed by cathechin. CONCLUSIONS: Catechin inhibited cataract-induced apoptotic cell death in the lens epithelium and may prove useful for the prevention of cataract progression.
