Protective Effects of Epigallocatechin Gallate after UV Irradiation in Cultured Human Retinal Pigment Epithelial Cells.
10.3341/kjo.2007.21.4.232
- Author:
Seong Won YANG
1
;
Byung Rae LEE
;
Jae Woong KOH
Author Information
1. Department of Ophthalmology, Chosun University College of Medicine, Gwangju, Korea. clearcornea@chosun.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Cellular activity;
Cultured human retinal pigment epithelial cells;
Epigallocatechin Gallate;
UV irradiation
- MeSH:
Antioxidants/*pharmacology;
Catechin/*analogs & derivatives/pharmacology;
Cell Count;
Cells, Cultured;
Dose-Response Relationship, Radiation;
Humans;
Pigment Epithelium of Eye/cytology/*drug effects/radiation effects;
Radiation Injuries/pathology/*prevention & control;
Radiation-Protective Agents;
Spectrophotometry;
*Ultraviolet Rays
- From:Korean Journal of Ophthalmology
2007;21(4):232-237
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: To evaluate the protective effects of Epigallocatechin gallate (EGCG) against UV irradiation in cultured human retinal pigment epithelial (RPE) cells. METHODS: UV irradiation was produced by a UV lamp for 30 seconds with an irradiance of 3.3 mW/cm2. After 5 minutes and 1 hour, we administered different concentrations of EGCG (0, 5, 10, 15, 25, 50, 100 uM). The cell count was determined under a microscope using a counting chamber and the cell activity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. RESULTS: The cell count of cultured human RPE cells after UV irradiation was markedly increased in the EGCG administration group, compared with the non-administrated group. The cell activity of the cultured human RPE cells after UV irradiation was markedly increased in the EGCG administration group and was increased in a dose-dependent way as determined by the MTT assay. CONCLUSIONS: The administration of EGCG increased the cell count and the cell activity after UV irradiation in cultured human retinal pigment epithelial cells; this suggests that EGCG provided protection against UV damage in cultured human retinal pigmented epithelial cells.
