Role of HGF/c-Met in Serum-Starved ARPE-19 Cells.
10.3341/kjo.2007.21.4.244
- Author:
Eun Jung JUN
1
;
Hwa Sun KIM
;
Yeong Hoon KIM
Author Information
1. Department of Ophthalmology, College of Medicine, The Catholic University of Korea, Seoul, Korea. kiwise@unitel.co.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
HGF/c-Met;
PVR;
RPE cells;
Starvation
- MeSH:
Blotting, Southern;
Blotting, Western;
Cell Movement/physiology;
Cell Proliferation;
Cells, Cultured;
Culture Media, Serum-Free;
*Gene Expression;
Hepatocyte Growth Factor/biosynthesis/*genetics;
Humans;
Mitosis/physiology;
Pigment Epithelium of Eye/cytology/*metabolism;
Polymerase Chain Reaction;
Proto-Oncogene Proteins c-met/biosynthesis/*genetics;
RNA/*genetics
- From:Korean Journal of Ophthalmology
2007;21(4):244-250
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Hepatocyte growth factor (HGF) and its receptor (HGFR/c-Met) regulate motility, mitogenesis, and morphogenesis in a cell type-dependent fashion. We report the role of HGF and c-Met on stress-induced ARPE-19 human retinal pigment epithelial (RPE) cells in this study. METHODS: The cells were cultured either with or without serum. Southern and Western blot analyses were done to determine the expression patterns of HGF/c-Met in serum-starved ARPE-19 cells. The cell proliferation pattern in serum-starved condition was analyzed using MTS assay. Inhibition level of cell proliferation was analyzed using a neutralizing monoclonal antibody against c-Met (2 microgram/ml). RESULTS: Abnormal cell proliferation and scattering of ARPE-19 cells was observed under serum starvation. HGF/c-Met were expressed in serum-starved ARPE-19 cells. ARPE-19 cell proliferation was also enhanced with recombinant HGF treatment. Neutralization against c-Met inhibited the proliferation of serum-deprived ARPE-19 by 64.5% (n=9, S.D. 5.5%). Serum starvation appears to induce epithelial-mesenchymal transition of ARPE-19 cells, resulting in scatter, and the expression of alpha-smooth muscle actin (alpha-SMA), a marker for fibrosis. CONCLUSIONS: In conclusion, c-Met induced under non-physiologic conditions has significant effects on the activation of RPE cells.
