Characteristics of nonalcoholic fatty liver disease induced in wistar rats following four different diets.
- Author:
Nicole FAKHOURY-SAYEGH
1
;
Viviane TRAK-SMAYRA
;
Aline KHAZZAKA
;
Fady ESSEILY
;
Omar OBEID
;
May LAHOUD-ZOUEIN
;
Hassan YOUNES
Author Information
- Publication Type:Original Article
- Keywords: Adiponectin; sucrose; triglycerides; fructose; Wistar rats
- MeSH: Adiponectin; Alanine Transaminase; Animals; Carbohydrates; Diet*; Fatty Liver*; Fructose; Humans; Liver; Male; Overnutrition; Prevalence; Rats; Rats, Wistar*; Sucrose; Triglycerides
- From:Nutrition Research and Practice 2015;9(4):350-357
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND/OBJECTIVES: The prevalence of nonalcoholic fatty liver disease (NAFLD) has increased worldwide in parallel with overnutrition characterized by high-fat and high-carbohydrate intake. Our objective was to establish, in 16 weeks, a model of NAFLD in Wistar pathogen-free rats following four dietary types. MATERIALS/METHODS: Forty (6 weeks old) healthy Wistar male rats, weighing an average of 150 g were randomly divided into four groups of ten and assigned a diet with the same quantity (15 g/rat/day), but with different composition. The moderate-fat (MF) group was fed a moderate-fat diet (31.5% fat and 50% carbohydrates), the high-fat (HF) group was fed a fat-rich diet (51% fat), the high-sucrose (HS) group and the high-fructose (HFr) group were fed a carbohydrate-rich diet (61%). The carbohydrate contents of the HS group was composed of 60.3% sucrose while that of the HFr group was composed of 59.3% fructose. RESULTS: At week 16, the HF group had the highest percentage of cells enriched in fat (40%) and the highest weight and liver weight (P < 0.05). The HFr group showed significantly higher levels of serum triglycerides, alanine aminotransferase and adiponectin at week 16 as compared to week 1 (P < 0.05). CONCLUSIONS: The 15 g/rat/day diet composed of 51% fat or 61% carbohydrates enriched mainly in fructose may induce characteristics of NAFLD in rats.
