Production and Characterization of Murine Monoclonal Antibodies ( MAbs ) which Specifically Recognize B-Subunit of Human Chorionic Gonadotropin ( HCG ).
- Author:
Heui Keun KANG
;
Jin Dong CHANG
;
Hyung Jun LEE
;
Jung Hak CHA
;
Moo Young SONG
;
In Young KO
- Publication Type:Original Article
- Keywords:
Human Chorionic Gonadotropin (HCG);
Monoclonal Antibody (Mab);
betahcg Specificity
- MeSH:
Animals;
Antibodies, Monoclonal*;
Blotting, Western;
Choriocarcinoma;
Chorionic Gonadotropin*;
Clone Cells;
Cloning, Organism;
Down Syndrome;
Enzyme-Linked Immunosorbent Assay;
Female;
Humans*;
Mass Screening;
Mice;
Pregnancy;
Sensitivity and Specificity
- From:Korean Journal of Immunology
1998;20(3):303-308
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
We have constructed several panels of MAbs which specifically recognize B-subunit of HCG (BHCG). Splenocytes from Balb/c mice immunized with B-subunit of HCG were fused with SP2/o-Ag14 myeloma cells by PEG method. Fifteen different hybridorna clones (individually named as mG10.127, mG10.61, mG9.5, mG9.18, rnG9.20, mG6.3, mG6.36, mG6.8, mG7.31, mG7.79, mG9.11, mG9.51.6, mG9.51.12, mH4.17, and mH4.4) were obtained by indirect ELISA screening and three to five successive cloning procedures. The distinct features of these MAbs were determined by specificity, western blot, isotyping, and isoelectrofocusing. All of the MAbs except mG9.20 and mG6.8 specifically bind to BHCG without cross- reaction with B-subunit of LH (BLH). In western blot analysis, all of the MAbs bind to non-denatured form of BHCG suggesting that the MAbs recognize conformation-dependent epitope of BHCG. This new panels of MAbs to BHCG should be useful for developing diagnostic reagent such as pregnancy, choriocarcinoma, Down's syndrome as well as for the fine quantitation of serum or urinary HCG.
