Generation and Characterization of Alloenic Radiation Bone Marrow Chimera.
- Author:
Ki Young LEE
;
Kil Hyoun KIM
- Publication Type:Original Article
- Keywords:
Allogenic chimera;
Tolerance;
Extrathymic development
- MeSH:
Animals;
Bone Marrow Cells;
Bone Marrow*;
Chimera*;
Humans;
Lymph Nodes;
Mice;
Phenotype;
Spleen;
Thymus Gland;
Tissue Donors
- From:Korean Journal of Immunology
1998;20(3):333-341
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Allogenic bone marrow chimera has been used to study the differentiation of donor-derived bone marrow cells under the recipient thymic environment, tolerance generaion between recipient and donor cells. We prepared H-2' to H-2 allogenic chimera by transfering bone marrow cells from H-2dmice to r-irradiated H-2k mice, and examined the differentiation ofthe bone marrow cells under allogenic environment. Complete reconstitution with H-2d+ phenotype cells in the thymus of the chimera mice was observed. However, the number of CD4- CD8+ cells dramatically decreased under the recipient thymic environment, CD8+ cells significantly reduced also in spleen and lymph node, compared with that of normal mice. Interestingly, we also observed coexistence of donor-derived cells (H-2k) and recipient derived cells (H-2d) in lymph node and spleen in the chimera. These results suggested that the decrease of CD4-CD8+ cells could be caused by r-irradiation by affecting the recipient thymic environment, and that in this chimera, tolerance between donor-derived cells and recipient-derived cells was maintained.
