Surface characteristics and bioactivity of an anodized titanium surface.
10.5051/jpis.2013.43.4.198
- Author:
Kyul KIM
1
;
Bo Ah LEE
;
Xing Hui PIAO
;
Hyun Ju CHUNG
;
Young Joon KIM
Author Information
1. Department of Periodontology, Dental Research Institute, Chonnam National University School of Dentistry, Gwangju, Korea. youngjun@chonnam.ac.kr
- Publication Type:Original Article
- Keywords:
Cell proliferation;
Dental implants;
Titanium;
Titanium oxide
- MeSH:
Alkaline Phosphatase;
Animals;
Cell Adhesion;
Cell Proliferation;
Cell Survival;
Corrosion;
Dental Implants;
Durapatite;
Mice;
Microscopy, Atomic Force;
Microscopy, Electron, Scanning;
Osteoblasts;
Photoelectron Spectroscopy;
Surface Properties;
Titanium
- From:Journal of Periodontal & Implant Science
2013;43(4):198-205
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: The aim of this study was to evaluate the surface properties and biological response of an anodized titanium surface by cell proliferation and alkaline phosphatase activity analysis. METHODS: Commercial pure titanium (Ti) disks were prepared. The samples were divided into an untreated machined Ti group and anodized Ti group. The anodization of cp-Ti was formed using a constant voltage of 270 V for 60 seconds. The surface properties were evaluated using scanning electron microscopy, X-ray photoelectron spectroscopy, and an image analyzing microscope. The surface roughness was evaluated by atomic force microscopy and a profilometer. The contact angle and surface energy were analyzed. Cell adhesion, cell proliferation, and alkaline phosphatase activity were evaluated using mouse MC3T3-E1 cells. RESULTS: The anodized Ti group had a more porous and thicker layer on its surface. The surface roughness of the two groups measured by the profilometer showed no significant difference (P>0.001). The anodized Ti dioxide (TiO2) surface exhibited better corrosion resistance and showed a significantly lower contact angle than the machined Ti surface (P>0.001). Although there was no significant difference in the cell viability between the two groups (P>0.001), the anodized TiO2 surface showed significantly enhanced alkaline phosphatase activity (P<0.001). CONCLUSIONS: These results suggest that the surface modification of Ti by anodic oxidation improved the osteogenic response of the osteoblast cells.
