Grape extracts suppress the formation of preneoplastic foci and activity of fatty acid synthase in rat liver.
- Author:
Sanghui KWEON
1
;
Yoon KIM
;
Haymie CHOI
Author Information
1. Department of Food and Nutrition, Seoul National University, San 56-1, Shillim-dong, Kwanak-gu, Seoul 151-742, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- MeSH:
Administration, Oral;
Animals;
Body Weight/drug effects;
Catalase/metabolism;
Cell Transformation, Neoplastic/*drug effects;
*Diet;
Dietary Supplements;
Fatty Acid Synthetase Complex/*metabolism;
Glutathione/metabolism;
Glutathione Peroxidase/metabolism;
Liver/*drug effects/enzymology/pathology;
Liver Neoplasms/diet therapy/prevention&control;
Male;
Organ Weight/drug effects;
Plant Extracts/*administration&dosage/*pharmacology;
Rats;
Rats, Sprague-Dawley;
Support, Non-U.S. Gov't;
Vitis/*chemistry
- From:Experimental & Molecular Medicine
2003;35(5):371-378
- CountryRepublic of Korea
- Language:English
-
Abstract:
This study was conducted to examine the effects of dietary grape extracts on preneoplastic foci formation in rat hepatocarcinogenesis, and related hepatic enzymes. Male Sprague-Dawley rats were fed basal diet or grape diet containing 15% concentrated grape extracts (68 bricks). The grape diet groups were divided into whole-period grape diet group (DEN-GW; grape diet group fed throughout experimental period) and postinitiation grape diet group (DEN-GP; grape diet group fed from post initiation stage) according to the starting time point of the grape diet. Hepatocarcinogenesis was induced by diethylnitrosamine (DEN; 200 mg/kg bw) and 2/3 partial hepatectomy (DEN-B; DEN-treated basal diet group, DEN-GW, and DEN-GP groups), while the control group treated with saline and sham operation (Control group). The formation of placental glutathione (GSH) S-transferase positive (GST-P(+)) foci in DEN-GW group was moderately but significantly suppressed, however, not in DEN- GP group. Thiobarbituric acid reactive substances content of DEN-GW group was significantly lower than that of DEN-B group. The activity of fatty acid synthase (FAS) in the grape diet groups was decreased about 1/2 of the DEN-B group. The content of GSH and GSH peroxidase activity were increased by carcinogen treatment, but not modulated by grape diet. The activities of GSH S-transferase, p-nitrophenol hydroxylase, and catalase were not affected by diet or treatment. Conclusively, the grape diet-induced reduction of FAS activity that was expressed highly in neoplastic tissues, might be one of the contributing mechanisms of hepatic cancer prevention.
