B cells activated in the presence of Th1 cytokines inhibit osteoclastogenesis.
- Author:
Youngnim CHOI
1
;
Jeong Jae KIM
Author Information
1. Department of Orofacial Infection and Immunity, College of Dentistry, Seoul National University, 28 Yungun-dong, Jongro-gu, Seoul 110-749, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- MeSH:
Animals;
B-Lymphocytes/cytology/*drug effects/immunology;
Base Sequence;
Cell Differentiation/*drug effects;
Cytokines/*pharmacology;
Female;
Giant Cells/cytology/drug effects;
Interferon Type II/immunology/metabolism;
Lymphocyte Activation/*drug effects;
Mice;
Molecular Sequence Data;
Osteoclasts/*cytology/*drug effects;
Phenotype;
Support, Non-U.S. Gov't;
Th1 Cells/*immunology;
Tumor Necrosis Factor/pharmacology
- From:Experimental & Molecular Medicine
2003;35(5):385-392
- CountryRepublic of Korea
- Language:English
-
Abstract:
Host immune response has been considered as an important disease-modifying factor of periodontitis, however, which immune cell(s) or factor(s) are involved in the destruction of periodontium remains unclear. Previously, we reported that osteoclastogenesis is enhanced by activated B cells but suppressed by activated CD8(+)T cells. We present new data that B cells activated in the presence of Th1 cytokines inhibit osteoclastogenesis. Purified murine B cells were activated with anti-IgD mAb, IL-4, and anti-CD40 mAb, in the absence (B(Th2)) or presence of Th1 cytokines, either IL-2 (B(IL-2)) or IFN-gamma (B(IFN-gamma)). Each activated B cell population was co-cultured with RAW264.7 cells in the presence of soluble receptor activator of NF-kappaB ligand (sRANKL), and the effect on osteoclastic differentiation was evaluated. While B(Th2)increased osteoclastogenesis, B(IL-2)and B(IFN-gamma)suppressed it profoundly. To verify the mediating molecule(s), we analyzed cytokine profiles of the activated B cells. Compared to B(Th2), B(IL-2)expressed increased amount of IFN-gamma and B(IFN-gamma)expressed decreased amounts of IL-4, IL-5, and IL-10. IFN-gamma was a key negative regulator of osteoclastic differentiation, and mediated the inhibition by B(IL-2). These results suggest that Th1 cytokines may have new important roles in resistance to periodontitis, acting directly on osteoclasts or indirectly through B cells.
