CD99 type II is a determining factor for the differentiation of primitive neuroectodermal cells.
- Author:
Eun Jung LEE
1
;
Hyun Gyu LEE
;
Sung Hye PARK
;
Eun Young CHOI
;
Seong Hoe PARK
Author Information
1. Department of Pathology, College of Medicine, Seoul National University, Seoul, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- MeSH:
Antigens, CD/genetics/*metabolism;
Bucladesine/pharmacology;
Cell Adhesion Molecules/genetics/*metabolism;
*Cell Differentiation/drug effects;
Cell Line;
Cell Size/drug effects;
Ectoderm/*cytology/drug effects/*metabolism;
Human;
Neurites/drug effects;
Neurons/*cytology/drug effects/*metabolism;
Protein Isoforms/genetics/metabolism;
Support, Non-U.S. Gov't;
Transfection
- From:Experimental & Molecular Medicine
2003;35(5):438-447
- CountryRepublic of Korea
- Language:English
-
Abstract:
CD99 is a 32-kDa cell surface molecule present on thymocytes, peripheral T cells, many other hematopoietic stem cells and somatic cells were implicated in cell-cell adhesion and cell-activation phenomena. Two major subtypes have been identified so far, designated CD99 type I and type II. We have investigated the correlation between the degree of neural differentiation and the expression of CD99 subtypes in three differentially differentiated cell lines such as CADO-ES1, RD-ES, and SH-N-SY5Y, in order of differentiation. In addition, we induced differentiation of the RD-ES cell line by N(6),2'-dibutyryl-cAMP (db-cAMP). Six days after treatment with db-cAMP, RD-ES cell line has changed its morphology from uniform round cells to cells with neurites, and initially CD99 type II-overexpressed RD-ES cells showed significant down-regulation of CD99 type II, whereas CD99 type I expression remained constant. When RD- ES cells were transfected with the cDNA encoding for CD99 type I-green fluorescence protein (GFP) and type II-GFP, CD99 type II transfected RD-ES cell line remained unchanged with morphology of undifferentiated form. Our data suggest that CD99 type II acts as a negative regulator in the neural differentiation of precursor cells that might occur during nerve system development.