Isolation, Characterization and Growth Kinetic Comparison of Bone Marrow and Adipose Tissue Mesenchymal Stem Cells of Guinea Pig.
10.15283/ijsc.2016.9.1.115
- Author:
Ghaem ALIBORZI
1
;
Akbar VAHDATI
;
Davood MEHRABANI
;
Seyed Ebrahim HOSSEINI
;
Amin TAMADON
Author Information
1. Department of Biology, Fars Science and Research Branch, Islamic Azad University, Fars, Iran.
- Publication Type:Original Article
- Keywords:
Mesenchymal stem cells;
Guinea pig;
Adipose tissue;
Bone marrow;
Characterization
- MeSH:
Adipocytes;
Adipose Tissue*;
Animals;
Bone Marrow*;
Cell- and Tissue-Based Therapy;
Guinea Pigs*;
Guinea*;
Mesenchymal Stromal Cells*;
Models, Animal;
Multipotent Stem Cells;
Osteoblasts
- From:International Journal of Stem Cells
2016;9(1):115-123
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Mesenchymal stem cells (MSCs) from different sources have different characteristics. Moreover, MSCs are not isolated and characterized in Guinea pig for animal model of cell therapy. AIM OF THE WORK: was the isolating of bone marrow MSCs (BM-MSCs) and adipose tissue MSCs (AT-MSCs) from Guinea pig and assessing their characteristics. MATERIAL AND METHODS: In this study, bone marrow and adipose tissue were collected from three Guinea pigs and cultured and expanded through eight passages. BM-MSCs and AT-MSCs at passages 2, 5 and 8 were seeded in 24-well plates in triplicate. Cells were counted from each well 1~7 days after seeding to determine population doubling time (PDT) and cell growth curves. Cells of passage 3 were cultured in osteogenic and adipogenic differentiation media. RESULTS: BM-MSCs and AT-MSCs attached to the culture flask and displayed spindle-shaped morphology. Proliferation rate of AT-MSCs in the analyzed passages was more than BM-MSCs. The increase in the PDT of MSCs occurs with the increase in the number of passages. Moreover, after culture of BM-MSCs and AT-MSCs in differentiation media, the cells differentiated toward osteoblasts and adipocytes as verified by Alizarin Red staining and Oil Red O staining, respectively. CONCLUSION: BM-MSCs and AT-MSCs of Guinea pig could be valuable source of multipotent stem cells for use in experimental and preclinical studies in animal models.
