Expression of Vascular Endothelial Growth Factor and iNOS in Pterygia.
- Author:
Kwang Ju LEE
1
;
Myung Jin JOO
;
Do Hyung LEE
Author Information
1. Department of Ophthalmology, Seoul Paik Hospital, Inje University, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Nitric oxide (NO);
Pterygia;
Vascular endothelial growth factor (VEGF)
- MeSH:
Conjunctiva;
Enzyme-Linked Immunosorbent Assay;
Epithelium;
Immunohistochemistry;
Nitric Oxide;
Vascular Endothelial Growth Factor A*
- From:Journal of the Korean Ophthalmological Society
1999;40(8):2087-2093
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
To evaluate the expression of vascular endothelial growth factor (VEGF) in pterygia and seek the reciprocal relationships between VEGF and nitric oxide (NO) in development of pterygia. Conjunctiva sampled during conjunc-tival transplantation of pterygial operation and pterygia were used in this study. OCT compound-fixed-cryopreserved tissues consisted normal conjunctiva and pterygia were used to study the expression of VEGF and iNOS with immunohistochemistry. For confirmation of NOS activity, NADPH-diaphorase staining was done. ELISA for detection of VEGF amounts was performed. The expression of VEGF and iNOS were revealed in the epithelium of pterygia, although there were not expressed in the epithelium of normal conjunctiva. The epithelium of pterygia was stained with NADPH-diaphorase. The results of ELISA showed the higher amount of VEGF inpterygia compared with conjunctiva. These findings suggest that VEGF and NO play an important role in fibrovascular development of pterygia.s
