Adenovirus Expressing Human Interferon Inhibits Replication of Foot and Mouth Disease Virus and Reduces Fatal Rate in Mice.
10.4167/jbv.2012.42.3.224
- Author:
Jia Qi CHU
1
;
Su Mi KIM
;
Kwang Nyeong LEE
;
Jae Ku OEM
;
Young Joon KO
;
Hyang Sim LEE
;
Yong Joo KIM
;
Jee Yong PARK
;
Kwang Jae KIM
;
Satya PARIDA
;
Yooni OH
;
David J PATON
;
Yi Seok JOO
;
Byounghan KIM
;
Jong Hyeon PARK
Author Information
1. Animal, Plant and Fisheries Quarantine and Inspection Agency (QIA), Anyang, Korea. parkjhvet@korea.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
FMD;
Interferon;
Protection;
Adenovirus
- MeSH:
Adenoviridae;
Adenoviruses, Human;
Animals;
Asia;
Enzyme-Linked Immunosorbent Assay;
Foot;
Foot-and-Mouth Disease;
Foot-and-Mouth Disease Virus;
Humans;
Interferon-alpha;
Interferon-beta;
Interferons;
Interleukin-18;
Kidney;
Livestock;
Mice;
Mice, Inbred ICR;
Survival Rate;
Thyroid Gland
- From:Journal of Bacteriology and Virology
2012;42(3):224-231
- CountryRepublic of Korea
- Language:English
-
Abstract:
Interferon is an important cytokine that plays a critical role in the initial host defense against viral infection. Recombinant human adenoviruses expressing human interferon-alpha (Ad-HIFNalpha) or pig interferon-beta fused with interleukin-18 (Ad-PIFNbeta-IL18) were constructed and used to induce an early protective response against foot and mouth disease (FMD). To analyze the antiviral effect, bovine thyroid and porcine kidney IBRS-2 cells and ICR mice were treated with Ad-HIFNalpha, Ad-PIFNbeta-IL18, and cocktail of Ad-HIFNalpha and Ad-PIFNbeta-IL18. The survival rate of suckling mice was monitored after foot and mouth disease virus (FMDV) challenge following intra-peritoneal (IP) administration of appropriate adenovirus. Indirect antigen ELISA was performed to evaluate inhibition of FMDV replication following challenge with the FMDV O, A, or Asia 1 serotypes in vitro. These recombinant adenoviruses reduced the replication of FMDV in susceptible cells, thereby decreasing the fatality in mice, suggesting that they can be a useful control method for the early protection against FMD infection in livestock after field trial.
