Cyclooxygenase expressions and response to radiation therapy in uterine cervix cancer.
- Author:
Yong Tark JEON
1
;
Sang Soo SEO
;
Jae Weon KIM
;
Noh Hyun PARK
;
Soon Beom KANG
;
Hyo Pyo LEE
;
Yong Sang SONG
Author Information
1. Department of Obstetrics and Gynecology, Cancer Research Institute, College of Medicine, Seoul National University, Seoul, Korea. yssong@snu.ac.kr
- Publication Type:Original Article
- Keywords:
Cervix cancer;
Cyclooxygenase;
Radiation
- MeSH:
Antibodies;
Blotting, Western;
Cell Line;
Cervix Uteri*;
Female;
Humans;
Prostaglandin-Endoperoxide Synthases*;
Radiotherapy;
Uterine Cervical Neoplasms
- From:Korean Journal of Gynecologic Oncology
2006;17(2):105-111
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: The aim of this study is to clarify the relationship between COX expressions and radioresistance in cervical cancer. METHODS: Patients with cervical cancer treated by primary radiotherapy were selected from the tumor registry of our institution. According to the response to radiotherapy during and after a month of radiation, poor responder and good responder was defined. Immunohistochemical staining was performed by the ABC method using formalin-fixed, paraffin-embedded tissue sections and monoclonal anti-COX-1,2 antibodies. Correlation of COX expression and response to radiation was analyzed. Cell lines derived from human cervical tumors were used: HeLa, HT3, and C33A. Using western blot, COX-1,2 expressions were identified in each cell line. The sensitivity of the cervix cancer cells to radiation was measured using a clonogenic assay. RESULTS: COX-1 and COX-2 expressions were higher in poor responders than good responders. The difference of COX-1 expression between two groups had marginal statistical significance (p=0.099, Fisher's exact test) and COX-2 expression was significantly higher in poor responders (p=0.034, Fisher's exact test). In the clonogenic assay, survival fraction of HeLa and HT-3 cell lines, which have COX-1 and COX-2 activity, was significantly higher than C33A cell line which has no COX activity (p<0.001). CONCLUSION: Our results suggest that the expression of COX in cervical cancer might be deeply associated with the effect of radiation therapy.
