The Enhancing Effect of Ethanol on the Development of Glutatione S-Transferase Placental Form-Positive Foci Induced by Diethylnitrosamine in F344 Rat.
- Author:
Kyung Ja CHO
1
;
Na Hye MYUNG
;
Ja June JANG
Author Information
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords: Ethanol; Porcine serum; DEN; GST-P; foci
- MeSH: Animals; Diethylnitrosamine/*toxicity; Ethanol/*pharmacology; Glutathione Transferase/*metabolism; Immune Sera/pharmacology; Liver Cirrhosis, Alcoholic/enzymology; Male; Placenta/drug effects/*enzymology; Precancerous Conditions/*chemically induced/enzymology; Rats; Rats, Inbred F344; Survival Rate; Swine
- From:Journal of Korean Medical Science 1990;5(1):13-17
- CountryRepublic of Korea
- Language:English
- Abstract: The effects of ethyl alcohol and pig serum administration on the development of preneoplastic hepatic enzyme-altered foci were examined in an in vivo mid-term assay system. Rats were initially given a single dose (200 mg/Kg) intraperitoneal injection of diethylnitrosamine (DEN). Two weeks later, treatment was started with 10% ethanol + 10% sucrose solution, 10% sucrose solution, or tap water as drinking water for 6 weeks with or without intraperitoneal injection of porcine serum twice a week. All rats were subjected to a two-thirds partial hepatectomy at week 3. The modification potentials were evaluated by comparing the number and area per cm2 of glutathione S-transferase placental form-positive (GST-P+) foci in the liver of each group. As a result, ethanol significantly enhanced the development of GST-P+ foci. Unfortunately, the porcine serum injection produced no hepatic fibrosis and no significant alteration in GST-P+ foci.
