Rat-Hepatocyte Culture and Differentiation in Hormone-Supplemented Media.
- Author:
Hee Cheol KIM
1
;
Jee Soo KIM
;
Sung Eun JUNG
;
Kuhn Uk LEE
Author Information
1. Department of Surgery, University of Ulsan College of Medicine and Asan Medical Center.
- Publication Type:Original Article
- Keywords:
Bioartificial liver system;
Hepatocyte culture;
Insulin;
EGF
- MeSH:
Ammonia;
Animals;
Culture Media;
Epidermal Growth Factor;
Hepatocytes;
Humans;
Insulin;
Liver Diseases;
Liver Transplantation;
Liver, Artificial;
Quality of Life;
Rats
- From:Journal of the Korean Surgical Society
1999;56(1):20-26
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Severe liver disease is very often life threatening and dramatically diminishes the quality of life. An effective artificial liver support system should be capable of sustaining patients with severe liver disease, preparing patients for liver transplantation, and improving the survival of and the quality of life for patients for whom transplantation is not a therapeutic options. METHODS: As an elemental experiment for establishing a bioartificial liver system, rat hepatocytes were isolated in several hormone-supplemented culture media: 1) control group, 2) insulin supplemented media, 3) EGF supplemented media, 4) insulin EGF supplemented media. Using primarily the cultured and subcultured hepatocytes, we evaluated the hepatic cellular function in each media. RESULTS: The cellular viability was maintained as long as 10 days (average, 8.4 days). The hepatocytes in the group 4 media showed good results in ammonia degradation, ureogenesis, and albumin synthesis compared with those in the other groups. In the subculture, the hepatocyte functions had significantly declined. CONCLUSION: Insulin EGF supplemented culture media were superior to the others.
