Recombinant tetra-cell adhesion motifs supports adhesion, migration and proliferation of keratinocytes/fibroblasts, and promotes wound healing.
- Author:
Mi Yeon JUNG
1
;
Narendra THAPA
;
Jung Eun KIM
;
Jung Duk YANG
;
Byung Chae CHO
;
In San KIM
Author Information
1. Department of Biochemistry and Cell Biology, Cell and Matrix Research Institute, School of Medicine, Kyungpook National UniversityDaegu 700-422, Korea. iskim@knu.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
betaIG-H3 protein;
cell adhesion;
cell movement;
cell proliferation;
fibronectin;
wound healing
- MeSH:
Amino Acid Motifs;
Animals;
Cell Adhesion/*drug effects;
Cell Line;
Cell Movement/*drug effects;
Cell Proliferation/*drug effects;
Extracellular Matrix Proteins/chemistry/genetics/pharmacology;
Fibroblasts/cytology/drug effects/physiology;
Fibronectins/chemistry/genetics/*pharmacology;
Humans;
Keratinocytes/cytology/drug effects/physiology;
Mice;
NIH 3T3 Cells;
Rabbits;
Recombinant Fusion Proteins/chemistry/genetics/pharmacology;
Transforming Growth Factor beta/chemistry/genetics/pharmacology;
Wound Healing/*drug effects/physiology
- From:Experimental & Molecular Medicine
2007;39(5):663-672
- CountryRepublic of Korea
- Language:English
-
Abstract:
An extracellular matrix protein plays an important role in skin wound healing. In the present study, we engineered a recombinant protein encompassing the 9th and 10th type III domains of fibronectin, and 4th FAS1 domain of beta ig-h3. This recombinant protein, in total, harbors four known-cell adhesion motifs for integrins: Pro-His-Ser-Arg-Asn (PHSRN) and Arg-Gly-Asp (RGD) in 9th and 10th type III domains of fibronectin, respectively, and Glu-Pro-Asp-Ile-Met (EPDIM) and Try-His (YH) in 4th FAS1 domain of big-h3, were designated to tetra-cell adhesion motifs (T-CAM). In vitro studies showed T-CAM supporting adhesion, migration and proliferation of different cell types including keratinocytes and fibroblasts. In an animal model of full-thickness skin wound, T-CAM exhibited excellent wound healing effects, superior to both 4th FAS1 domain of beta ig-h3 or 9th and 10th type III domains of fibronectin. Based on these results, T-CAM can be applied where enhancement of cell adhesion, migration and proliferation are desired, and it could be developed into novel wound healing drug.
