Salinomycin inhibited cell proliferation and induced apoptosis in human uterine leiomyoma cells.
10.5468/ogs.2014.57.6.501
- Author:
Hyun Gyo LEE
1
;
Ji Min LEE
;
So Jin SHIN
;
Sang Hoon KWON
;
Gi Su LEE
;
Chang Ho SONG
;
Eun Som CHOI
;
Soon Do CHA
;
Chi Heum CHO
Author Information
1. Institute for Cancer Research, Keimyung University School of Medicine, Daegu, Korea. chcho@kmu.ac.kr
- Publication Type:Original Article
- Keywords:
Apoptosis;
Extrinsic;
Intrinsic;
Leiomyoma;
Salinomycin
- MeSH:
Apoptosis*;
Blotting, Western;
Caspase 3;
Cell Death;
Cell Proliferation*;
Cell Survival;
DNA Fragmentation;
Down-Regulation;
Humans;
Leiomyoma*
- From:Obstetrics & Gynecology Science
2014;57(6):501-506
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: The aim of this study was to investigate the anti-proliferative effect of the salinomycin in cell proliferation and apoptosis in primary cultured human uterine leiomyoma cells. METHODS: Cell viability was measured by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Caspase-3 activity assay and DNA fragmentation assay were performed to determine the effect of apoptosis. The expression of apoptosis regulatory-related proteins was evaluated by western blot. RESULTS: The cell viability and proliferation of uterine leiomyoma cells were significantly reduced by salinomycin treatment in a dose-dependent manner. DNA fragmentation assay results showed apoptotic cell death after salinomycin incubation. Salinomycin activated caspase-3, -8, and -9, causing apoptosis in uterine leiomyoma cells. Down-regulation of Bcl-2, XIAP, and FLIP with a concomitant increase in Bax, Fas, and DR5 were observed. CONCLUSION: These results provided the first evidence that salinomycin induce both intrinsic and extrinsic apoptosis. Therefore, salinomycin may be a promising chemopreventive and therapeutic agent against human uterine leiomyoma.
