Circulating Aneuploid Cells Detected in the Blood of Patients with Infectious Lung Diseases.
10.5090/kjtcs.2017.50.2.126
- Author:
Hongsun KIM
1
;
Jong Ho CHO
;
Chung Hee SONN
;
Jae Won KIM
;
Yul CHOI
;
Jinseon LEE
;
Jhingook KIM
Author Information
1. Department of Thoracic and Cardiovascular Surgery, Sungkyunkwan University School of Medicine, Korea. jkimsmc@skku.edu
- Publication Type:Case Report
- Keywords:
Circulating neoplastic cells;
Fluorescent in situ hybridization;
Lung neoplasms
- MeSH:
Aneuploidy*;
Chromosome Aberrations;
Communicable Diseases;
Diagnosis;
Empyema;
Epithelial Cells;
Filtration;
Fluorescence;
Fluorescent Antibody Technique;
Humans;
In Situ Hybridization;
In Situ Hybridization, Fluorescence;
Lung Diseases*;
Lung Neoplasms;
Lung*;
Neoplastic Cells, Circulating
- From:The Korean Journal of Thoracic and Cardiovascular Surgery
2017;50(2):126-129
- CountryRepublic of Korea
- Language:English
-
Abstract:
The identification of circulating tumor cells (CTCs) is clinically important for diagnosing cancer. We have previously developed a size-based filtration platform followed by epithelial cell adhesion molecule immunofluorescence staining for detecting CTCs. To characterize CTCs independently of cell surface protein expression, we incorporated a chromosomal fluorescence in situ hybridization (FISH) assay to detect abnormal copy numbers of chromosomes in cells collected from peripheral blood samples by the size-based filtration platform. Aneuploid cells were detected in the peripheral blood of patients with lung cancer. Unexpectedly, aneuploid cells were also detected in the control group, which consisted of peripheral blood samples from patients with benign lung diseases, such as empyema necessitatis and non-tuberculous mycobacterial lung disease. These findings suggest that chromosomal abnormalities are observed not only in tumor cells, but also in benign infectious diseases. Thus, our findings present new considerations and bring into light the possibility of false positives when using FISH for cancer diagnosis.
