Skin Organ Culture Model for Evaluation of Melanin Pigmentation.
- Author:
Joong Sun LEE
1
;
Ji Yeoun LEE
;
You Mi CHOI
;
Yong Sik JUNG
;
Won Hyoung KANG
;
Hee Young KANG
Author Information
1. Department of Dermatology, Ajou University School of Medicine, Suwon, Korea. i4kk@yahoo.com
- Publication Type:Original Article
- Keywords:
Skin organ culture;
Pigmentation
- MeSH:
Biology;
Melanins*;
Melanocytes;
Organ Culture Techniques*;
Pigmentation*;
Skin Diseases;
Skin*
- From:Korean Journal of Dermatology
2005;43(4):450-454
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Skin organ culture is widely used as a tool to investigate skin biology or skin disease. OBJECTIVE: The objective of the present study was to develop an ideal skin organ culture model for evaluation of melanin pigmentation. METHODS: An air-liquid interface and submerged method were used. The histology of the cultured skin was studied with H&E stain. To examine the epidermal pigmentation, Fontana-Masson stain and NKI/beteb stain were performed. Pigment modifiers (arbutin, LY294002) were applied to the culture medium for 3 days as an air-liquid interface culture. RESULTS: The general architecture of the skin was well maintained for 5 days. The melanin pigment decreased during culture without change of the number of melanocytes. As expected from previous reports, the effect of pigment modifiers (arbutin, LY294002) on cultured skin was demonstrated. CONCLUSION: The results indicate that this skin organ culture model is useful in evaluating the melanin pigmentation
