Removal efficacy and cytotoxicity of a calcium hydroxide paste using N-2-methyl-pyrrolidone as a vehicle.
10.5395/rde.2017.42.4.290
- Author:
Myung Jin LIM
1
;
Hyun Jin JANG
;
Mi Kyung YU
;
Kwang Won LEE
;
Kyung San MIN
Author Information
1. Department of Conservative Dentistry, Chonbuk National University School of Dentistry and Institute of Oral Bioscience, Jeonju, Korea. mksdd@jbnu.ac.kr
- Publication Type:Original Article
- Keywords:
Calcium hydroxide;
Cytotoxicity;
N-methylpyrrolidone;
Removal
- MeSH:
Agar;
Bicuspid;
Calcium Hydroxide*;
Calcium*;
Cell Survival;
Dental Pulp Cavity;
Ointments;
Polyethylene Glycols;
Propylene Glycol;
Tooth
- From:Restorative Dentistry & Endodontics
2017;42(4):290-300
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVES: This study investigated the removal efficacy and cytotoxicity of a newly developed calcium hydroxide paste (cleaniCal, Maruchi) using N-2-methyl-pyrrolidone (NMP) as a vehicle in comparison with ApexCal (Ivoclar Vivadent) and Calcipex II (Nishika), which use different vehicles such as polyethylene glycol and propylene glycol, respectively. MATERIALS AND METHODS: Thirty maxillary premolars with oval-shaped canals were divided into 3 groups and the teeth were filled with one of the pastes. After removal of the paste, micro-computed tomographic (μ-CT) imaging was obtained to assess the volume of residual paste in the root canal of each tooth. The teeth were then split longitudinally and the area of the paste-coated surface was evaluated by stereomicroscopy. The cytotoxicity of each product was assessed using an agar overlay assay. The effect of each vehicle on cell viability was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The data were analyzed using one-way analysis of variance and Tukey's tests to detect any significance (p < 0.05). RESULTS: In the μ-CT and stereomicroscopic analysis, cleaniCal exhibited less remnants of medicament than ApexCal and Calcipex. cleaniCal showed a higher cytotoxicity than the other pastes in the agar overlay assay. Furthermore, NMP exhibited lower cell viability compared to the other vehicles. CONCLUSIONS: cleaniCal showed better removal efficacy compared to the other products. However, clinicians should be aware of the higher cytotoxicity of the NMP-based material and consider its possible adverse effects on periradicular tissue when it is overfilled.
