Comparison of apical extrusion of intracanal bacteria by various glide-path establishing systems: an in vitro study.
10.5395/rde.2017.42.4.316
- Author:
Alberto DAGNA
1
;
Rashid EL ABED
;
Sameeha HUSSAIN
;
Ibrahim H ABU-TAHUN
;
Livia VISAI
;
Federico BERTOGLIO
;
Floriana BOSCO
;
Riccardo BELTRAMI
;
Claudio POGGIO
;
Hyeon Cheol KIM
Author Information
1. Department of Clinical, Surgical, Diagnostic and Pediatric Sciences, School of Dentistry, Endodontic Unit, University of Pavia, Pavia, Italy.
- Publication Type:Original Article
- Keywords:
Bacterial extrusion;
Debris extrusion;
Glide-path;
Manual instrumentation;
Nickel-titanium rotary file
- MeSH:
Agar;
Bacteria*;
Brain;
Enterococcus faecalis;
Ethylene Oxide;
Glass;
Heart;
In Vitro Techniques*;
Molar;
Stem Cells;
Tooth
- From:Restorative Dentistry & Endodontics
2017;42(4):316-323
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVES: This study compared the amount of apically extruded bacteria during the glide-path preparation by using multi-file and single-file glide-path establishing nickel-titanium (NiTi) rotary systems. MATERIALS AND METHODS: Sixty mandibular first molar teeth were used to prepare the test apparatus. They were decoronated, blocked into glass vials, sterilized in ethylene oxide gas, infected with a pure culture of Enterococcus faecalis, randomly assigned to 5 experimental groups, and then prepared using manual stainless-steel files (group KF) and glide-path establishing NiTi rotary files (group PF with PathFiles, group GF with G-Files, group PG with ProGlider, and group OG with One G). At the end of canal preparation, 0.01 mL NaCl solution was taken from the experimental vials. The suspension was plated on brain heart infusion agar and colonies of bacteria were counted, and the results were given as number of colony-forming units (CFU). RESULTS: The manual instrumentation technique tested in group KF extruded the highest number of bacteria compared to the other 4 groups (p < 0.05). The 4 groups using rotary glide-path establishing instruments extruded similar amounts of bacteria. CONCLUSIONS: All glide-path establishment instrument systems tested caused a measurable apical extrusion of bacteria. The manual glide-path preparation showed the highest number of bacteria extruded compared to the other NiTi glide-path establishing instruments.
