Rh C/c, E/e genotyping by polymerase chain reaction using sequence-specific primers.
- Author:
Dong Hee SEO
1
;
Heung Bum OH
;
Yoo Sung HWANG
;
Sang In KIM
Author Information
1. Blood Transfusion Research Institute, The Republic of Korea National Red Cross.
- Publication Type:Original Article
- Keywords:
Rh C/c;
E/e;
Genotyping;
PCR-SSP
- MeSH:
Blood Donors;
Blood Grouping and Crossmatching;
DNA;
Fetus;
Genotype;
Humans;
Models, Genetic;
Phenotype;
Polymerase Chain Reaction*;
Serotyping
- From:Korean Journal of Blood Transfusion
1999;10(1):21-26
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUNDS: With the recent elucidation of genetic basis of Rh blood group, it is now available the molecular genotyping methods for Rh blood typing. These can be used when serological typing is difficult. This study was conducted to investigate the usefulness of Rh genotyping method for Koreans. METHODS: Genotyping for Rh C/c and E/e was performed in peripheral blood DNA samples from 34 blood donors by polymerase chain reaction using sequence-specific primers (PCR-SSP). The PCR determined genotypes were compared with serologically determined phenotypes. RESULTS: The Rh C/c and E/e genotyping results of 34 blood donors were full concordance with the results of their serologic phenotyping. CONCLUSIONS: Rh genotyping method on the basis of Rh genetic model can be applied to Koreans. This genotyping method would be useful tool in prenatal Rh typing of fetus at risk of hemolytic disease and when serotyping is not available for example massive transfusion.
