Isolation and characterization of equine amniotic membrane-derived mesenchymal stem cells.
10.4142/jvs.2013.14.2.151
- Author:
Min Soo SEO
1
;
Sang Bum PARK
;
Hyung Sik KIM
;
Jun Gu KANG
;
Joon Seok CHAE
;
Kyung Sun KANG
Author Information
1. Adult Stem Cell Research Center, Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea. kangpub@snu.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
amnion;
amniotic membrane;
equine;
isolation;
mesenchymal stem cells
- MeSH:
Adipogenesis;
Amnion/*cytology/physiology;
Animals;
*Cell Differentiation;
*Cell Lineage;
Cell Proliferation;
Chondrogenesis;
Female;
Flow Cytometry/veterinary;
Horses;
Immunophenotyping/veterinary;
Mesenchymal Stromal Cells/*cytology/physiology;
Osteogenesis
- From:Journal of Veterinary Science
2013;14(2):151-159
- CountryRepublic of Korea
- Language:English
-
Abstract:
Recent studies have shown that mesenchymal stem cells (MSCs) are able to differentiate into multi-lineage cells such as adipocytes, chondroblasts, and osteoblasts. Amniotic membrane from whole placenta is a good source of stem cells in humans. This membrane can potentially be used for wound healing and corneal surface reconstruction. Moreover, it can be easily obtained after delivery and is usually discarded as classified waste. In the present study, we successfully isolated and characterized equine amniotic membrane-derived mesenchymal stem cells (eAM-MSCs) that were cultured and maintained in low glucose Dulbecco's modified Eagle's medium. The proliferation of eAM-MSCs was measured based on the cumulative population doubling level (CPDL). Immunophenotyping of eAM-MSCs by flow cytometry showed that the major population was of mesenchymal origin. To confirm differentiation potential, a multi-lineage differentiation assay was conducted. We found that under appropriate conditions, eAM-MSCs are capable of multi-lineage differentiation. Our results indicated that eAM-MSCs may be a good source of stem cells, making them potentially useful for veterinary regenerative medicine and cell-based therapy.
