A Prospective Multicenter Study on the Etiological Analysis of Community-Acquired Pneumonia in Adult Patients in Korea: Detection of Mycoplasma pneumoniae and Chlamydia pneumoniae Infections.
- Author:
Cheol Hyun JOO
1
;
Ha Jung YOON
;
Jung Hyun NAM
;
Mi Sun MOON
;
Young Keol CHO
;
Jun Hee WOO
;
Wan Shik SHIN
;
Min Ja KIM
;
Hoan Jong LEE
;
Yoo Kyum KIM
Author Information
1. Department of Microbiology, College of Medicine, University of Ulsan.
- Publication Type:Multicenter Study ; Original Article
- Keywords:
Community-acquired pneumonia;
Mycoplasma pneumoniae;
Chlamydia pneumoniae
- MeSH:
Adult*;
Base Sequence;
Chlamydia*;
Chlamydophila pneumoniae*;
Humans;
Korea*;
Molecular Epidemiology;
Multiplex Polymerase Chain Reaction;
Mycoplasma pneumoniae*;
Mycoplasma*;
Pneumonia*;
Pneumonia, Mycoplasma*;
Polymerase Chain Reaction;
Polymorphism, Restriction Fragment Length;
Prevalence;
Prospective Studies*
- From:Korean Journal of Infectious Diseases
2001;33(1):15-23
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The purpose of this study is to develop rapid and sensitive method to detect M. pneumoniae and C. pneumoniae. In addition, we prospectively investigate the prevalence of M. pneumoniae and C. pneumoniae infections in community-acquried pneumonia. METHODS: The nasopharyngeal aspirates were collected from July 1997 to April 2000. PCR or nested PCR techniques were used to detect causative agents. RFLP and nucleotide sequence analysis were performed to study molecular epidemiology. Furthermore, feasibility of one-step technique to detect two microorganisms was tested by use of multiplex PCR. RESULTS: Of 250 clinical specimens, 23 (9.2%) specimens were positive for M. pneumoniae and 33 (13.2%) for C. pneumoniae, including one dual-infected specimen. Most M. pneumoniae infection occurred during the colder months of the year. C. pneumoniae infection was predominently found between December to February. CONCLUSION: The result indicate that M. pneumoniae and C. pneumoniae infections are frequently found from patients with community-acquired pneumoniae.
