Effect of immobilization stress on the expression of TH, BDH and CRH gene in rat brain.
- Author:
Yong Ri QIAN
1
;
Yoon Sik KIM
Author Information
1. College of Nursing Yanbian University, No. 39 Jiankang Road, Yanji city Lilin Prov, China.
- Publication Type:Original Article
- Keywords:
Immobilization stress;
TH;
DBH and CRH mRNAs
- MeSH:
Adrenal Glands;
Animals;
Blotting, Western;
Brain*;
Catecholamines;
Corticotropin-Releasing Hormone;
Immobilization*;
Rats*;
RNA, Messenger;
Sympathetic Nervous System;
Tyrosine 3-Monooxygenase
- From:Journal of Genetic Medicine
2007;4(2):179-185
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Catecholamines are the neuro-transmitters in the sympathetic nervous system (SNS) and are activated by stress stimulus. Tyrosine hydroxylase (TH) and Dopamine-beta-Hydroxylase (DBH) are very important enzymes in the catecholamine synthesis. Corticotropin releasing hormone (CRH) is released in the process of reacting to stresses. The aim of this study is to find out what effects immobilization stresses have on the expression of TH, BDH and CRH mRNA in a rat's brains. METHODS: We compare expression levels in rat's brains of TH, DBH and CRH mRNA induced by immobilization stresses between the test group and controled group. The expression levels of TH, DBH and CRH mRNA are measured by RT-PCR and the Western Blotting Analysis (WBA). RESULTS: In brains and adrenal glands of the immobilization stress group, the expression levels of TH and DBH mRNAs are significantly two to three times higher (P<0.01), and CRH mRNAs are approximately one and a half times higher (P<0.05) than those of controlled group. CONCLUSION: This study suggest that the expression levels of TH, DBH and CRH mRNAs are activated by stress stimulus in a rat's brains and adrenal glands.
