Molecular Biology of Cutaneous Fungal Infection.
- Author:
Jong Soo CHOI
1
Author Information
1. Department of Dermatology, College of medicine,Yeungnam University, Daegu, Korea. jschoi@med.yu.ac.kr
- Publication Type:Review
- Keywords:
Fungi;
PCR;
rDNA;
Molecular identification;
Molecular typing
- MeSH:
Diagnosis;
DNA;
DNA, Ribosomal;
Fungi;
Molecular Biology*;
Molecular Typing;
Polymerase Chain Reaction;
Polymorphism, Restriction Fragment Length
- From:Hanyang Medical Reviews
2006;26(4):52-58
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Traditional identification of fungi is based on their gross morphology and microscopic findings. It takes a long time and needs experience and sometimes it is difficult because their morphology may change. Nucleic acid detection methods such as PCR have become a common tool for microbial identification and diagnosis. Molecular typing techniques provide information to detect their infection routes. They are fast, sensitive, and reproducible. Pure DNA, the targets, and methods are important for good results. There are lots of techniques like sequencing, RFLP, RAPD, and hybridization. Each method has its advantage and limitation, and proper selection is essential. In clinical application, careful consideration will reduce errors. There is a limited number of reports about cutaneous fungal infection. We review the molecular methods for fungal identification and typing, and discuss the advantages and limitations of the methods.
