Multiplex Ligation Dependent Probe Amplification (MLPA) Assay on Joseon Mummified Samples from Archaeological Sites of South Korea.
10.11637/kjpa.2015.28.3.137
- Author:
You Soo KIM
1
;
Chang Seok OH
;
Jong Ha HONG
;
Moon Woo SEONG
;
Dong Hoon SHIN
Author Information
1. Department of Anatomy and Cell Biology, Seoul National University College of Medicine, Seoul, Korea. cuteminjae@gmail.com
- Publication Type:Original Article
- Keywords:
Mummification;
MLPA;
Korea
- MeSH:
DNA;
Humans;
Korea*;
Multiplex Polymerase Chain Reaction*;
Mummies
- From:Korean Journal of Physical Anthropology
2015;28(3):137-143
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Multiplex Ligation dependent Probe Amplification (MLPA) to detect large deletions or duplications has been widely used as a diagnostic tool for various disease clinically. As this method requires only a small amount of template DNA and is very simple and high throughput, it has numerous advantages for the analysis of the human specimen obtained from archaeological sites. In this study we therefore tried to perform MLPA analysis for detecting any of duplications or deletions in mummy samples (n=4) from medieval Joseon tombs of Korea. Of them, we could not get any authentic data from 3 samples by MLPA method while only one case (HD2) showed the possible presence of duplications or deletions during her lifetime. Although the current report reveal that MLPA is a promising tool for anthropological study in South Korea, more studies are still needed to make up for the validity problem of commercial MLPA kit used in this study.
