Effects of Curcumin and Genistein on Phorbol Ester or Tumor Necrosis Factor-alpha-Induced Mucin Production from Human Airway Epithelial Cells.
10.4046/trd.2011.70.3.218
- Author:
Su Yel LEE
1
;
Hyun Jae LEE
;
Jae Woo LEE
;
Byeong Kyou JEON
;
Ju Ock KIM
;
Choong Jae LEE
Author Information
1. Department of Pharmacology, Chungnam National University College of Medicine, Daejeon, Korea. LCJ123@cnu.ac.kr
- Publication Type:Original Article
- Keywords:
Mucin 5AC;
Curcumin;
Genistein
- MeSH:
Curcumin;
Epithelial Cells;
Genistein;
Humans;
Mucin 5AC;
Mucins;
Necrosis;
Phorbols;
Tumor Necrosis Factor-alpha
- From:Tuberculosis and Respiratory Diseases
2011;70(3):218-223
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: We investigated whether curcumin and genistein affect the MUC5AC mucin production from human airway epithelial cells that is induced by phorbol 12-myristate 13-acetate (PMA) or tumor necrosis factor-alpha (TNF-alpha). METHODS: Confluent NCI-H292 cells were pretreated with each agent for 30 min and then stimulated with PMA or TNF-alpha for 24 hours. MUC5AC mucin production was measured by an ELISA. RESULTS: (1) Curcumin dose-dependently inhibited the production of MUC5AC mucin that was induced by PMA or TNF-alpha; (2) Genistein inhibited PMA-induced MUC5AC mucin production. However, it did not decrease TNF-alpha-induced MUC5AC mucin production. CONCLUSION: These results suggest that curcumin and genistein inhibit the production of airway mucin induced by PMA.
