- Author:
Jong Wook KIM
1
;
Pyung Rim CHUNG
;
Myung Ki HWANG
;
Eun Young CHOI
Author Information
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords: Trichomonas vaginalis virus; TVV; dsRNA-dependent RNA polymerase; Totiviridae; ribosomal frameshift
- MeSH: Abscess/parasitology/pathology; Animals; Capsid Proteins/genetics; Cloning, Molecular; Disease Models, Animal; Female; Frameshifting, Ribosomal; Giardiavirus/classification/genetics/*isolation & purification; Humans; Korea; Mice; Molecular Sequence Data; Open Reading Frames; Phylogeny; RNA Replicase/genetics; RNA, Double-Stranded/*genetics; RNA, Viral/*genetics; Sequence Analysis, DNA; Sequence Homology; Trichomonas Infections/*virology; Trichomonas vaginalis/genetics/isolation & purification/pathogenicity/*virology; Virulence
- From:The Korean Journal of Parasitology 2007;45(2):87-94
- CountryRepublic of Korea
- Language:English
- Abstract: In this study, we describe Korean isolates of Trichomonas vaginalis infected with double-stranded (ds) RNA virus (TVV). One T. vaginalis isolate infected with TVV IH-2 evidenced weak pathogenicity in the mouse assay coupled with the persistent presence of a dsRNA, thereby indicating a hypovirulence effect of dsRNA in T. vaginalis. Cloning and sequence analysis results revealed that the genomic dsRNA of TVV IH-2 was 4,647 bp in length and evidenced a sequence identity of 80% with the previously-described TVV 1-1 and 1-5, but only a 42% identity with TVV 2-1 and 3 isolates. It harbored 2 overlapping open reading frames of the putative capsid protein and dsRNA-dependent RNA polymerase (RdRp). As previously observed in the TVV isolates 1-1 and 1-5, a conserved ribosomal slippage heptamer (CCUUUUU) and its surrounding sequence context within the consensus 14-nt overlap implied the gene expression of a capsid protein-RdRp fusion protein, occurring as the result of a potential ribosomal frameshift event. The phylogenetic analysis of RdRp showed that the Korean TVV IH-2 isolate formed a compact group with TVV 1-1 and 1-5 isolates, which was divergent from TVV 2-1, 3 and other viral isolates classified as members of the Giardiavirus genus.