Identification of streptococcus dysgalactiae subsp. equisimilis from septic knee by 16S rRNA gene sequencing.
- Author:
Ji Sang PARK
1
;
Hye Won KIM
;
Jeong Don CHAE
;
Jin Wuk HUR
;
Yoon Seok PARK
;
Min Seok YOO
;
Hee Yoon RYU
Author Information
- Publication Type:Case Report
- Keywords: Equisimilis; Infectious arthritis; Streptococcus dysgalactiae subsp.; 16S rRNA gene sequencing
- MeSH: Anti-Bacterial Agents; Arthritis; Arthritis, Infectious; Bacteria; Base Sequence; Female; Genes, rRNA*; Hemolysis; Humans; Joints; Knee*; Methods; RNA, Ribosomal, 16S; Streptococcus agalactiae; Streptococcus*
- From:Kosin Medical Journal 2016;31(1):79-85
- CountryRepublic of Korea
- Language:English
- Abstract: Septic arthritis is the infection of a joint by an infectious agent, leading to arthritis. It is therefore important to identify and treat the correct bacteria in septic arthritis. However, accurate identification of bacteria by conventional methods is difficult because of the distinct biochemical characteristics of individual bacteria. This case report aims at assessing septic arthritis caused by Streptococcus dysgalactiae subsp. equisimilis(SDSE) using nucleotide sequences and discusses the associated treatment. Here, Streptococcus agalactiae was determined to be the causative bacteria for septic arthritis in a 77 year-old woman using the conventional method of hemolysis pattern interpretation and morphology. However, nucleotide sequence analysis of 16S ribosomal RNA revealed that SDSE was the causative strain. 16S rRNA gene sequencing can correctly identify bacteria strains that are difficult to be identified by traditional method, and this correct identification can provide patients with the opportunity for adequate treatment using the proper antibiotics.
