Molecular Epidemiological Characteristics of Drug-resistant Mycobacterium tuberculosis by Random Amplified Polymorphic DNA Genotyping.
- Author:
Jong Chan JEON
1
;
Dong Seok JEON
;
Jae Ryong KIM
Author Information
1. Department of Family Medicine, Kyungju-Dongsan Hospital, Kyungju, Korea.
- Publication Type:Original Article
- Keywords:
Mycobacterium tuberculosis;
MDR;
RAPD;
Genotyping
- MeSH:
Discrimination (Psychology);
DNA*;
Korea;
Mycobacterium tuberculosis*;
Mycobacterium*;
Prevalence;
Tuberculosis
- From:Korean Journal of Clinical Pathology
2001;21(4):274-281
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: In spite of appropriate therapy and control for tuberculosis, the prevalence of tuberculosis is still frequent in Korea. Emerging infection and rapid detection of multidrug-resistant Mycobacterium tuberculosis (MDR-TB) are major interests in microbiologic laboratories. In this study, we evaluated the usefulness of random amplified polymorphic DNA (RAPD) genotyping for molecular epidemiological characteristics of MDR-TB. METHODS: We analyzed 64 clinical strains of M. tuberculosis including 35 strains which showed resistance to one or more antimycobacterial drugs and M. tuberculosis H37Rv (ATCC 27294), as a drug-sensitive control strain. RAPD genotyping analysis was carried out under eight reaction conditions and using ten random primers (A-1245, AP-50, B-1245, DKU-44, DKU-49, Leg-1, INS-2, IS-986-FP, PF-15 and MBR). RESULTS: RAPD patterns using six primers (IS-986-FP, DKU-44, DKU-49, INS-2, B-1245, and AP-50) showed marked polymorphisms that were easier to discriminate than those with other primers. RAPD patterns represented various polymorphisms among 64 strains. However, RAPD could not discriminate MDR-TB strains from drug-sensitive ones. CONCLUSIONS: RAPD genotyping is assumed a preferable technique for discrimination among clinical strains of M. tuberculosis but not for specifying MDR-TB strains.
