measurement of Percutaneous Absorption Using Fluorescence Polarization Immunoassays in a Diffusion Chamber.
- Author:
Weon Ju LEE
1
;
Yong Hyun KIM
;
Do Won KIM
;
Jae Bok JUN
;
Sang Lip CHUNG
Author Information
1. Department of Dermatology, Kyungpook National University School of Medicine, Taegu, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
Fluorescence polarization immunoassay;
Percutaneous absorption
- MeSH:
Absorption;
Acetone;
Diffusion*;
Epidermis;
Fluorescence Polarization Immunoassay*;
Fluorescence Polarization*;
Fluorescence*;
Histological Techniques;
Humans;
Lidocaine;
Skin;
Skin Absorption*;
Tissue Donors
- From:Korean Journal of Dermatology
1996;34(5):718-724
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The penetraton in vivo of topically applied substances can be assessed by physiological or pharmacalogical signs or analysed by chemical or histological techniques. In vitro absorption can be commonly quantitated by measuring the passage of a radioisotope-labelled substance across skin that has been mounted in a diffusion chamber. OBJECTIVE: Fluorescence polarization immunoassay technique has made the possible rapid growth of therapeutic drug nonitoring. We applied this methodology in measuring percutaneous absorption in a diffusion chamber. METHODS: We utilized sheets of whole epidermis prepared from the circumcised prepuce. Some epidermal sheets were treated with 2 ml of acetone for 2 minutes, and others not. The epidermal sheet was mounted in a diffusion chamber between the donor compartment for the penetrant and the receptor compartment containing saline. Lidocaine HC1(10 microgram/cm2) in vehicle(propylene glycol:ethanol; 7:3, vol/vol) was applied to the donor compartment for the penetrant. With flow rate of about 3 ml/h all of the receptor phase collected during 2 hours interval were quantitated for 10 hours by the fluorescence polarization immunoassay. RESULTS: Total absorption of lidocaine HC1 in the acetone-untreated group was 2.14+/-0.74% of the applied dose. Total absorption in the acetone-treated group showed no substantial difference (2.09+/-1.25%) compared to those of acetone-untreated group. The amount of lipid extracted from a epiderrnal sheet with acetone was 19+/-2.97%. CONCLUSION: Fluorescence polarization immunoassay may be a useful method in measuring percutaneous absorption in vitro.
